CENPW antibody (N-Term)

Catalog No. ABIN1587944

Product Details anti-CENPW Antibody

Target: CENPW (Centromere Protein W (CENPW))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPW antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: At least three isoforms of CENPW are known to exist, this antibody will detect all three isoforms.

Purification: Affinity chromatography purified via peptide column

Immunogen: CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW.

Isotype: IgG

Application Details

Application Notes: CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.

Comment:

(NT) Centromere protein W

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 0.02 % Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: 4 °C

Storage Comment: CENPW antibody can be stored at 4 °C for three months. At -20 °C stable for up to one year.

Expiry Date: 12 months

Target Details for CENPW

Target: CENPW (Centromere Protein W (CENPW))

Alternative Name: CENPW (CENPW Products)

Synonyms: C6orf173 antibody, CUG2 antibody, 2610036L11Rik antibody, Cug2 antibody, CENP-W antibody, centromere protein W antibody, CENPW antibody, Cenpw antibody

Background: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.

Gene ID: 387103

NCBI Accession: NP_001012525