LIF antibody

Catalog No. ABIN1589881

Product Details anti-LIF Antibody

Target: LIF (Leukemia Inhibitory Factor (LIF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIF antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recombinant human LIF

Characteristics: Chromosomal location: 22q12.2
Produced from sera of rabbits immunized with highly pure recombinant human Leukemina Inhibitory Factor (LIF) [Ser23 - Phe202, produced in E. coli].

Purification: Anti-human LIF was purified by affinity chromatography with immobilized Protein A.

Isotype: IgG

Application Details

Application Notes: Western Blot: use 2-5 µg/mL

Comment:

Rabbit IG Polyclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C

Expiry Date: 6 months

Target Details for LIF

Target: LIF (Leukemia Inhibitory Factor (LIF))

Alternative Name: LIF (LIF Products)

Synonyms: CDF antibody, DIA antibody, HILDA antibody, MLPLI antibody, LIF, interleukin 6 family cytokine antibody, leukemia inhibitory factor antibody, LIF antibody, Lif antibody

Background: Leukemia Inhibitory Factor also called LIF is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Human and mouse LIF exhibit a 78% identity in its amino acid sequence. Human LIF is as active on human cells as is it is on mouse cells, though mouse LIF is about 1000 fold less active on human cells, than human LIF.
Synonyms: LIF, CDF, DIA, HILDA, MLPLI

NCBI Accession: NP_002300, NM_002309

UniProt: P15018

Pathways: JAK-STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding