GREM1 antibody

Catalog No. ABIN1589903

Product Details anti-GREM1 Antibody

Target: GREM1 (Gremlin 1 (GREM1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GREM1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Recombinant human Gremlin-1

Characteristics: Chromosomal location: 15q13.3
Produced from sera of rabbits pre-immunized with highly pure (>95%) recombinant human Gremlin-1 (Lys25-Asp1848) derived from E. Coli.

Isotype: IgG

Application Details

Application Notes: Western Blot: use 2-5 µg/mL

Comment:

Rabbit IG Polyclonal Antibody

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Handling Advice: Centrifuge vial prior to opening.

Storage: -20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8 °C. Frozen aliquots are stable for at least 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for GREM1

Target: GREM1 (Gremlin 1 (GREM1))

Alternative Name: Gremlin-1 (GREM1 Products)

Synonyms: grem1 antibody, MGC136702 antibody, zgc:136702 antibody, GREM1 antibody, drm antibody, pig2 antibody, dand2 antibody, ihg-2 antibody, gremlin antibody, Cktsf1b1 antibody, Drm antibody, Grem antibody, ld antibody, gremlin-1 antibody, CKTSF1B1 antibody, DAND2 antibody, DRM antibody, GREMLIN antibody, IHG-2 antibody, cktsf1b1 antibody, gremlin 1a, DAN family BMP antagonist antibody, gremlin 1, DAN family BMP antagonist antibody, gremlin 1 antibody, gremlin 1, DAN family BMP antagonist L homeolog antibody, grem1a antibody, GREM1 antibody, LOC662541 antibody, grem1 antibody, Grem1 antibody, grem1.L antibody

Background: Gremlin, also known as “Increased in High Glucose protein 2” (IHG2) and “Down regulated in Mos-transformed cells protein” (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins. Native human Gremlin consist of 160 amino acids. The mature region contains one potential site for N-linked glycosylation (Asn42), a cysteine-rich region, and a cysteine-knot motif (aa94-184) whose structure is shared by members of the TGFβ superfamily. Posttranslational modifications include glycosylation and phosphorylation. Human Gremlin exists in both secreted and membrane-associated forms and there exist 2 isoforms. The aa sequence identity of human Gremlin with mouse and chicken Gremlin is 99% and 86 %, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle. Gremlin functions as a bone morphogenetic protein (BMP) antagonist. It acts by binding to, and forming heterodimers with, BMP2, BMP4, and BMP7, thus preventing them from interacting with their cell surface receptors. This mechanism is thought to be responsible for the pattern-inducing activity of Gremlin during embryonic development and to play a role in human diseases, such as diabetic nephropathy. However, intracellular BMP-independent mechanisms of action may mediate the ability of Gremlin to suppress transformation and tumor genesis under certain experimental conditions. Gremlin also interacts with Slit proteins and acts as an inhibitor of monocyte chemotaxis. In addition, Gremlin has been found to be a proangiogenic factor expressed by endothelium. Furthermore Gremlin is a novel agonist of the major proangiogenic receptor VEGFR2.
Synonyms: GREM1, DRM, PIG2, DAND2, IHG-2, GREMLIN, CKTSF1B1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, BMP antagonist 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, Increased in high glucose protein 2

NCBI Accession: NP_037504, NM_013372

UniProt: O60565

Pathways: Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location