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Mouse anti-Salmon Ig (Heavy Chain) Antibody (FITC)

FACS Monoclonal IPA5F12 IgG2a FITC
Catalog No. ABIN161600
  • Target See all Ig products
    Ig
    Binding Specificity
    • 76
    • 71
    • 56
    • 32
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Heavy Chain
    Reactivity
    • 110
    • 51
    • 24
    • 18
    • 9
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Salmon
    Host
    • 94
    • 82
    • 25
    • 18
    • 4
    • 1
    • 1
    Mouse
    Clonality
    • 115
    • 102
    Monoclonal
    Conjugate
    • 48
    • 24
    • 23
    • 23
    • 16
    • 16
    • 11
    • 8
    • 4
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    FITC
    Application
    • 154
    • 133
    • 90
    • 60
    • 47
    • 26
    • 23
    • 13
    • 11
    • 8
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS)
    Specificity
    Salmonid Ig (H chain)
    Characteristics
    The FITC anti-salmonid Ig (H chain) monoclonal antibody can be used to indi- rectly detect a generalized or pathogen-specific increase in immunoglobulin level. This antibody recognizes the immunoglobin heavy chain found in all Salmonid species (Salmo, Oncorhynchus and Salvelinus). This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in a FACS assay, followed by samples of infected fish serum and then the anti-salmonid Ig monoclonal antibody. This antibody can be used in Flow Cytometry.
    Purification
    Immunoaffinity chromatography
    Immunogen
    affinity purified Rainbow trout and Atlantic salmon heavy and light chain immunoglobulin . Fusion Partner: X63Ag8.653 myloma cells
    Clone
    IPA5F12
    Isotype
    IgG2a
  • Application Notes
    This antibody can be used as a detection reagent for fish infection by either detecting a generalized increase in fish immunoglobulin level or by detecting specific antibody. For specific antibody detection, known infectious agents are used as the antigen in a FACS assay, followed by samples of infected fish serum and then the anti-salmonid Ig monoclonal antibody. This antibody can be used in Flow Cytometry.
    Comment

    Excitation/Emission wavelength: 494 nm/514 nm

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.1 mg/mL
    Handling Advice
    Product is photosensitive and should be protected from light.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 4°C. For long term storage aliquot and freeze unused portion at -20C in volumes appropriate for single usage. Avoid freeze/thaw cycles.
  • Target
    Ig
    Abstract
    Ig Products
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