GATA2 antibody (pSer401) (HRP)

Catalog No. ABIN1712589

Product Details anti-GATA2 Antibody (HRP)

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Binding Specificity: pSer401

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA2 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human GATA2 around the phosphorylation site of Ser401

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA2 (GATA2 Products)

Synonyms: DCML antibody, MONOMAC antibody, NFE1B antibody, Gata-2 antibody, xgata-2 antibody, xgata2 antibody, GATA-2 antibody, NF-E1b antibody, GATA2 antibody, Gata2 antibody, gata2 antibody, zg2 antibody, zgc:91840 antibody, GATA binding protein 2 antibody, GATA binding protein 2 L homeolog antibody, amidase antibody, glutamyl/aspartyl-tRNA(Gln/Asp) amidotransferasesubunit A antibody, hypothetical protein antibody, glutamyl-tRNA(Gln) amidotransferase, subunit GatA (Glu-ADT subunit A) antibody, GATA2 transcription factor antibody, putative gata binding factor antibody, GATA binding protein 2a antibody, GATA binding protein 2b antibody, GATA2 antibody, Gata2 antibody, gata2.L antibody, RR_RS20245 antibody, gatA2 antibody, Smp_018590 antibody, gata2a antibody, gata2b antibody

Background:

Synonyms: GATA2 phospho S401, p-GATA2 phospho S401, GATA-2, GATA 2, GATA Binding Protein 2, GATA-binding protein 2, Gata2, GATA2_HUMAN, MGC2306, NFE 1B, NFE1B.

Background: Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary, lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites.

Gene ID: 2624

Pathways: Stem Cell Maintenance