GATA2 antibody (N-Term)

Catalog No. ABIN2777458

This Rabbit Polyclonal antibody specifically detects GATA2 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse and Rabbit. It has been mentioned in 6+ publications

Quick Overview for GATA2 antibody (N-Term) (ABIN2777458)

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GATA2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-GATA2 Antibody

Binding Specificity: N-Term

Purpose: GATA2 Antibody - N-terminal region

Sequence: PYYANPAHAR ARVSYSPAHA RLTGGQMCRP HLLHSPGLPW LDGGKAALSA

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against GATA2. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human GATA2

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8101364

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-GATA2 antibody (ABIN2777458)

Wang, Zhu, Guo, Dong, Liu, Yin, Zhang, Li, Liu, Ma, Song, He, Wang, Wang, Zhang, Li, Yu: "A regulatory circuit comprising GATA1/2 switch and microRNA-27a/24 promotes erythropoiesis." in: Nucleic acids research, Vol. 42, Issue 1, pp. 442-57, (2013) (PubMed).

Fujiwara, Yokoyama, Okitsu, Kamata, Fukuhara, Onishi, Fujimaki, Takahashi, Ishizawa, Bresnick, Harigae: "Gene expression profiling identifies HOXB4 as a direct downstream target of GATA-2 in human CD34+ hematopoietic cells." in: PLoS ONE, Vol. 7, Issue 9, pp. e40959, (2012) (PubMed).

Jack, Crossley: "GATA proteins work together with friend of GATA (FOG) and C-terminal binding protein (CTBP) co-regulators to control adipogenesis." in: The Journal of biological chemistry, Vol. 285, Issue 42, pp. 32405-14, (2010) (PubMed).

Huang, Niu, Liu, Wang, Sturgis, Wei: "The functional IGFBP7 promoter -418G>A polymorphism and risk of head and neck cancer." in: Mutation research, Vol. 702, Issue 1, pp. 32-9, (2010) (PubMed).

Mammoto, Connor, Mammoto, Yung, Huh, Aderman, Mostoslavsky, Smith, Ingber: "A mechanosensitive transcriptional mechanism that controls angiogenesis." in: Nature, Vol. 457, Issue 7233, pp. 1103-8, (2009) (PubMed).

Tsuzuki, Kitajima, Nakano, Glasow, Zelent, Enver: "Cross talk between retinoic acid signaling and transcription factor GATA-2." in: Molecular and cellular biology, Vol. 24, Issue 15, pp. 6824-36, (2004) (PubMed).

Target Details for GATA2

Target: GATA2 (GATA Binding Protein 2 (GATA2))

Alternative Name: GATA2

Background:

Background Information: The GATA family of transcription factors, which contain zinc fingers in their DNA binding domain, have emerged as candidate regulators of gene expression in hematopoietic cells is essential for normal primitive and definitive erythropoiesis and is expressed at high levels in erythroid cells, mast cells, and megakaryocytes. GATA2 is expressed in hematopoietic progenitors, including early erythroid cells, mast cells, and megakaryocytes, and also in nonhematopoietic embryonic stem cells.

Gene Name: GATA binding protein 2

Alternative Symbols: DCML, IMD21, NFE1B, MONOMAC

Protein Name: Endothelial transcription factor GATA-2

Molecular Weight: 51 kDa

Gene ID: 2624

NCBI Accession: NP_116027

UniProt: P23769

Pathways: Stem Cell Maintenance