Cathelicidin antibody (AA 31-170) (Biotin)

Catalog No. ABIN1175686

Product Details anti-Cathelicidin Antibody (Biotin)

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Binding Specificity: AA 31-170

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathelicidin antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- QVLSYKEAVL RAIDGINQRS SDANLYRLLD LDPRPTMDGD PDTPKPVSFT VKETVCPRTT QQSPEDCDFK KDGLVKRCMG TVTLNQARGS FDISCDKDNK RFALLGDFFR KSKEKIGKEF KRIVQRIKDF LRNLVPRTES

Specificity: It has been selected for its ability to recognize CAMP in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant CAMP expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against CAMP conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant CAMP (Gln31~Ser170) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for Cathelicidin

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Abstract: CAMP Products

Synonyms: CAP-18 antibody, CAP18 antibody, CRAMP antibody, FALL-39 antibody, FALL39 antibody, LL37 antibody, CATH-2 antibody, Cnlp antibody, Cramp antibody, MCLP antibody, CATH-B1 antibody, CATHB1 antibody, CATHL2 antibody, CATHL7 antibody, CAP11 antibody, cathelicidin antimicrobial peptide antibody, CAMP antibody, LOC100136187 antibody, LOC100136439 antibody, Camp antibody

Pathways: Cellular Response to Molecule of Bacterial Origin