Interleukin 8 antibody (IL8)

Details for Product anti-IL8 Antibody No. ABIN1724413, Supplier: Log in to see
Antigen
  • IL8
  • K60
  • interleukin-8
  • il8
  • si:dkey-151b16.2
  • cxcli2
  • Il8
  • Scyb15
  • lungkine
  • weche
  • CXCL8
  • GCP-1
  • GCP1
  • LECT
  • LUCT
  • LYNAP
  • MDNCF
  • MONAP
  • NAF
  • NAP-1
  • NAP1
  • IL-8
  • AMCF-I
  • EMF-1
  • interleukin 8-like 1
  • interleukin 8
  • chemokine (C-X-C motif) ligand 1-like
  • chemokine (C-X-C motif) ligand 15
  • interleukin 8-like 2
  • interleukin-8-like
  • IL8L1
  • il8
  • IL8
  • LOC422654
  • Cxcl15
  • Il8
  • IL8L2
  • IL*08-02
Alternatives
anti-Human Interleukin 8 antibody for ELISA
Reactivity
Human
432
31
24
20
19
15
14
10
8
7
6
5
4
4
4
1
1
1
1
1
Host
Mouse
323
222
9
6
6
5
2
Clonality
Monoclonal
Conjugate
This Interleukin 8 antibody is un-conjugated
60
47
16
11
7
5
5
5
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
380
355
128
108
40
35
34
32
29
23
23
15
13
12
10
6
5
5
4
2
2
2
2
2
2
1
1
1
1
Options
Supplier
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Supplier Product No.
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'Independent Validation' Badge
  • Successfully validated (Immunohistochemistry)
  • by  HIK Hannover
  • No. #100789
  • Date 05/31/2017
  •  
Antigen IL8
Method validated Immunohistochemistry
Positive Control Formalin fixed and plastic embedded human liver, testicle, and tonsil tissue sections
Negative Control No primary antibody
Notes Passed. The IL8 antibody ABIN1724413 specifically labels the targeted antigen in human liver, testicle, and tonsil sections in IHC. No signal was detected in tonsil negative control tissue sections and the no primary antibody negative controls.
Primary Antibody ABIN1724413
Secondary Antibody Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608)
Protocol
  • Disect tissue and fix in 4% buffered formalin for >24h at RT.
  • Plastification in Technovit 9100 plastic embedding system (Heraeus Kulzer) according to manufactuer’s recommencations.
  • Sectioning into 5µm sections on a rotary microtome (Reichert Jung, 1140/Autocut).
  • Deplastify and rehydrate tissue through graded xylene and graded alcohol series:
    • xylene for 20min.
    • MEA (2-Methoxyethyl)acetat zur Synthese (Merck, 8.06061.1000) 2x 20min.
    • 100% ethanol for 1min.
    • 95% ethanol for 1min.
    • 70% ethanol for 1min.
    • H2O for 2min.
    • distilled H2O 2min.
  • Heat induced epitope retrieval in high pH Trilogy buffer (Medac Diagnostika, 920P-07, lot 1622912A) ON at 60°C in an oven (Memmert, UNB 200).
  • Quench sections in in ice cold distilled H2O 2min.
  • Adjust sections with wash buffer (Medac Diagnostika, B1-30A, lot no 161102001) 5min at RT.
  • Incubate sections with primary Interleukin 8 antibody (antibodies-online, ABIN1724413) diluted 1:400 in dilution buffer (Medac Diagnostika, B1-31C, lot no 161205001) for 30min at RT. Includeno primaryantibody negative controls.
  • Rinse sections 3x 5min with wash buffer. Keep negative controls in a separate container.
  • Block endogenous peroxidase with Peroxide Block RTU (Medac Diagnostika, 925B-09, lot no 161103001) for 10min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Histofine Simple Stain MAX PO (M) Anti-Mouse (Medac Diagnostika, 414132F, lot no H1608) for 30min at RT.
  • Rinse sections 3x 5min with wash buffer.
  • Incubate sections with Bright DAB (Medac Diagnostika, BS04-110, lot no 160523) for 10min at RT.
  • Rinse sections in distilled H2O.
  • Counterstain with Mayers Haematoxylin (Leica, 3801582E, lot no3801582E) 5min at RT.
  • Dehydrate in fresh ethanol:
    • 95% ethanol twice for 2min.
    • 99.5% ethanol twice for 2min.
    • Xylene twice for 2min.
  • Mount sections with Celltexx mounting medium (Engelbrecht, 19360, lot no 1716047).
  • Dry sections in a ventilated place.
  • Image acquisition on a Leica Microsystems DM 1000 light microscope with a DFC 320 digital camera. Magnification tonsil sections 40x, liver sections 10x and 20x, testicle sections 10x.
Experimental Notes Immunostaining pattern of human liver, testicle, and tonsil with ABIN1724413 is consistent with published data.
Validation Images
Immunohistochemistry validation image for anti-Interleukin 8 (IL8) antibody (ABIN1724413) Immunostaining of IL8 in human liver liver (A), testicle (C), and tonsil sections (D)...
Immunogen Recombinant protein corresponding to full length IL8
Isotype IgG1
Specificity Reacts with human IL-8
Purification Purified
Alternative Name IL-8 (IL8 Antibody Abstract)
Background IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively.
Gene ID 3576
Research Area Cytokines, Immunology, Virology, Inflammation, Cancer
Pathways Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy
Application Notes

Working dilution: Optimal dilution should be determined by the end user.
The following are guidelines only:
IHC-P (5 μg/mL) WB (1:1000)

Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Citrate-Tris-HCl buffer, pH 7.0, 0.02 % Proclin 300
Preservative ProClin
Precaution of Use This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
Supplier Images
 image for anti-Interleukin 8 (IL8) antibody (ABIN1724413) anti-Interleukin 8 (IL8) antibody
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