Eph Receptor tyrosine Kinase A2 (EPHA2) antibody

Antigen: Eph Receptor tyrosine Kinase A2 (EPHA2)

Clonality: Monoclonal (Kalpha-5H5)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA), Immunofluorescence (IF)

Catalog no.: ABIN179724

Additional Information

Alternative name: Eph receptor tyrosine kinase A2 (EphA2)

Immunogen: genetic immunisation with cDNA encoding human EphA2

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: Kalpha-5H5

Description: EphA2 (Eph receptor tyrosine kinase A2) belongs to the Eph tyrosine receptor familiy, the largest receptor tyrosine kinase family of transmembrane proteins. It encodes a 130 kDA transmembrane protein which is primarily found in adult human epithelial cells (1). Eph receptors and their ephrin ligands are important mediators of cell-cell communication and play roles in embryonic patterning, neuronal targeting, and vascular development during normal embryogenesis (2,3). The Eph family of receptor tyrosine kinases is frequently overexpressed in a wide variety of cancers and tumor cell lines. In particular, EphA2 is overexpressed in prostate, lung and colon cancers and 40% of breast cancers and it represent an attractive potential target for drug design (3,4).

Specificity: Anti-human EphA2

Application Details

Application Notes: FACS: 1.2ug/10^6 cells, Cell-ELISA: 1:200 to 1:400, Immunofluorescence: 1 ug/10^6 cells

Concentration: 2 mg/ml

Purification: Protein G

Buffer: phosphate buffered saline, pH 7.2

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using Kalpha-5H5. BOSC23 cells were transiently trans-fected with an expression vector encoding either EphA2(red curve) or an irrelevant protein (control transfectant).Binding of Kalpha-5H5 was detected with a PE-conjugatedsecondary antibody. A positive signal was obtained onlywith EphA2 transfected cells.

Spectral Confocal Microscopy of CHO cells using K?-5H5. CHO cells were transiently transfected with an expression vector encoding EphA2. Binding of K?-5H5 was visualized with a FITC-conjugated secondary antibody (green). Actin filaments are labeled with

SDS-PAGE analysis of purified K?-5H5 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified K?-5H5 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain Reagent.

Publications

Publications: Lindberg, Hunter: "cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6316-24, 1991 (PubMed).

Nakamoto, Bergemann: "Diverse roles for the Eph family of receptor tyrosine kinases in carcinogenesis." in: Microscopy research and technique, Vol. 59, Issue 1, pp. 58-67, 2002 (PubMed).

Brantley-Sieders, Schmidt, Parker et al.: "Eph receptor tyrosine kinases in tumor and tumor microenvironment." in: Current pharmaceutical design, Vol. 10, Issue 27, pp. 3431-42, 2004 (PubMed).

Ireton, Chen: "EphA2 receptor tyrosine kinase as a promising target for cancer therapeutics." in: Current cancer drug targets, Vol. 5, Issue 3, pp. 149-57, 2005 (PubMed).