Kallikrein-Related Peptidase 3 (KLK3) antibody

Antigen: Kallikrein-Related Peptidase 3 (KLK3)

Synonyms: APS, PSA, hK3, KLK2A1, KLK3, KLKB1

Clonality: Monoclonal (LT-3D2)

Host: Mouse

Reactivity: Human

Application: Flow Cytometry (FACS), Cell-ELISA (cELISA)

Catalog no.: ABIN179726

Additional Information

Alternative name: Prostate-Specific Antigen (PSA)

Immunogen: genetic immunisation with cDNA encoding human PSA

Format: Purified

Isotype: IgG1  (Matching secondary antibodies)

Clone: LT-3D2

Description: Prostate specific antigen (PSA) belongs to the kallikrein gene family. It is a single-chain glycoprotein with a molecular mass of about 33 kD which is produced by prostate epithelial cells. It is secreted in the seminal plasma and is responsible for liquefaction of the seminal coagulum (1,2). PSA is present in small quantities in the serum of healthy males, and is often elevated in the presence of prostate cancer and in other prostate disorders. It is one of the most useful tumour markers because of its tissue specificity. It is thus widely used for screening, diagnosis, and monitoring patients with prostate cancer (3).

Specificity: Anti-human PSA (glandular kallikrein 3)

Application Details

Storage: Short term: 2°C - 8°C. long term: -20°C (avoid repeated freezing and thawing)

Restrictions: For Research Use only

Images

FACS analysis of BOSC23 cells using LT-3D2. BOSC23 cells were transiently transfected with anexpression vector encoding either PSA (red curve) or an irrelevant protein (control transfectant: black curve). Binding ofLT-3D2 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with PSA transfected cells.

SDS-PAGE analysis of purified LT-3D2 monoclonal antibody. Lane 1: molecular weight marker, Lane 2: 2 ug of purified LT-3D2 antibody. Proteins were separated by SDS-PAGE and stained with RAPID Stain Reagent.

Publications

Publications: Oesterling: "Prostate specific antigen: a critical assessment of the most useful tumor marker for adenocarcinoma of the prostate." in: The Journal of urology, Vol. 145, Issue 5, pp. 907-23, 1991 (PubMed).

Balk, Ko, Bubley: "Biology of prostate-specific antigen." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 21, Issue 2, pp. 383-91, 2003 (PubMed).

Lilja: "Biology of prostate-specific antigen." in: Urology, Vol. 62, Issue 5 Suppl 1, pp. 27-33, 2003 (PubMed).