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Prostate Specific Antigen antibody (PSA) Primary Antibody

PSA Reactivity: Human DB, FACS, ELISA, WB Host: Mouse Monoclonal A5D5-1 unconjugated
Independent Validation (1)
Catalog No. ABIN1543584
$725.79
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1 mg
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  • Target See all Prostate Specific Antigen (PSA) Antibodies
    Prostate Specific Antigen (PSA)
    Reactivity
    • 252
    • 15
    • 12
    • 6
    • 4
    • 1
    • 1
    Human
    Host
    • 142
    • 92
    • 14
    • 4
    • 3
    Mouse
    Clonality
    • 154
    • 100
    Monoclonal
    Conjugate
    • 128
    • 24
    • 17
    • 10
    • 8
    • 6
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Prostate Specific Antigen antibody is un-conjugated
    Application
    • 112
    • 82
    • 45
    • 44
    • 39
    • 33
    • 32
    • 15
    • 14
    • 12
    • 12
    • 10
    • 8
    • 7
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Dot Blot (DB), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
    Purification
    Protein A affinity column
    Immunogen
    Native PSA Antigen
    Clone
    A5D5-1
    Isotype
    IgG1
  • Comment

    Other Applications have not been tested.

    Restrictions
    For Research Use only
  • Validation #029617 (Flow Cytometry)
    'Independent Validation' Badge
    by
    Flow Cytometry & Cell Separation Facility, Purdue University
    No.
    #029617
    Date
    02/22/2014
    Antigen
    Lot Number
    FAPS-A5D5-0003
    Method validated
    Flow Cytometry
    Positive Control
    LNCaP cells
    Negative Control
    - DU145 cells - Jurkat cells
    Notes
    Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
    'Independent Validation' Badge
    Validation Images
    Full Methods
    Primary Antibody
    • Antibody: Prostate Specific Antigen (PSA)
    • Catalog number: ABIN1543584
    • Lot number: FAPS-A5D5-0003
    Secondary Antibody
    • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
    Full Protocol
    • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
    • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
    • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
    • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
    • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
    • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
    • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
    • Cells were washed twice with PBS/BSA/Tween 20
    • Cells were resuspended in 0.3 mL PBS/BSA
    • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
    Experimental Notes
    None
  • Format
    Liquid
    Concentration
    1.8 mg/mL
    Buffer
    PBS, pH 7.4, 0.09% sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
  • Target
    Prostate Specific Antigen (PSA)
    Alternative Name
    PSA (PSA Products)
    Synonyms
    APS, KLK2A1, PSA, hK3, KLK3, Klk1c10l2, RSGK-50, RSKG-50, rGK-3, Kal-3, Kal3, Klk3, AAP-S, MP100, Psa, R74825, goku, kallikrein related peptidase 3, kallikrein 3, kallikrein 1-related peptidase C3, kallikrein B, plasma 1, aminopeptidase puromycin sensitive, KLK3, Klk1c3, Klkb1, Npepps
    Pathways
    Complement System
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