Prostate Specific Antigen antibody (PSA)

Details for Product anti-PSA Antibody No. ABIN1543584
Antigen
Reactivity
Human
619
15
8
5
1
1
1
Host
Mouse
454
132
28
7
7
Clonality (Clone)
Monoclonal ()
Conjugate
This Prostate Specific Antigen antibody is un-conjugated
36
29
18
16
11
11
5
5
5
5
5
5
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Dot Blot (DB), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
365
186
128
117
99
73
47
32
32
28
26
25
14
10
7
6
6
6
4
4
3
2
1
1
1
1
Options
Immunogen Native PSA Antigen
Clone A5D5-1
Isotype IgG1
Purification Protein A affinity column
Antigen
Alternative Name PSA (PSA Antibody Abstract)
Pathways Complement System
Comment

Other Applications have not been tested.

Restrictions For Research Use only
Format Liquid
Concentration 1.8 mg/mL
Buffer PBS, pH 7.4, 0.09% sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
'Independent Validation' Badge
Lot Number FAPS-A5D5-0003
Method validated Flow Cytometry
Positive Control LNCaP cells
Negative Control - DU145 cells - Jurkat cells
Notes Strong signal above isotype signal was observed in the positive control sample, while staining of similar level to isotype control was observed in both negative control samples.
Primary Antibody
  • Antibody: Prostate Specific Antigen (PSA)
  • Catalog number: ABIN1543584
  • Lot number: FAPS-A5D5-0003
Isotype Control Antibody
  • Antibody: mouse IgG1
Secondary Antibody
  • Antibody: F(ab’)2 goat anti-mouse IgG-Alexa 647
Controls
  • Positive control: LNCaP prostate tumor cell line
  • Negative control: DU145 prostate tumor cell line, Jurkat T cell leukemia cell line
  • Isotype control: cells treated with mouse IgG1 instead of the primary antibody to confirm that primary antibody binding is specific.
Protocol
  • Positive and negative control cells were washed once with phosphate-buffered saline (PBS) and harvested with 0.05% trypsin / 0.025% EDTA.
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37°C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA and 0.1% Tween 20
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA.
  • 1 µg of anti-PSA or mouse IgG1 isotype control were added to the cells and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA/Tween 20
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA
  • Secondary antibody was added at a final dilution of 1:500 and incubated for 30 min at room temperature
  • Cells were washed twice with PBS/BSA/Tween 20
  • Cells were resuspended in 0.3 mL PBS/BSA
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a red laser (640 nm excitation / 660 nm emission).
Experimental Notes None
Validation Images
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 1: LNCaP cells stained with anti-PSA (blue) or with isotype control (red).
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 2: DU145 cells stained with anti-PSA (blue) or with isotype control (red).
Flow Cytometry validation image for anti-Prostate Specific Antigen (PSA) antibody (ABIN1543584) Figure 3: Jurkat cells stained with anti-PSA (blue) or with isotype control (red).