GM-CSF antibody

Catalog No. ABIN180855

Product Details anti-GM-CSF Antibody

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GM-CSF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Functional Studies (Func)

Specificity: When immobilised on a microplate, this antibody will capture recombinant and natural GM-CSF.

Characteristics: Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulatingfactor, Colony stimulating factor 2

Purification: Protein A chromatography, 0.2µm sterile filtered

Immunogen: R-DNA. Genename: GM-CSF

Clone: 30-04-14

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on frozen sections: used at 25g/mLELISA: 1/1K - 1/2K. Western Blot: used at 1-2g/mLFunctional Assays: The neutralisation dose (ND50) of this batch was determined to beapproximately 0.3-0.5 μg/mL in the presence of 0.5 ng/mL of recombinant human GM-CSF. ND50 may vary depending on conditions. ND50 is defined as the concentration of Abrequired to yield half maximal inhibition of cytokine activity on a responsive cell line, whenthat cytokine is present at a concentration just high enough to elicit a maximum response.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.2 with 1 % Trehalose

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GM-CSF

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Alternative Name: GM-CSF (CSF2 Products)

Synonyms: GMCSF antibody, GM-CSF antibody, CSF2 antibody, Csfgm antibody, Gm-CSf antibody, MGI-IGM antibody, Gm-csf antibody, Gmcsf antibody, CSF antibody, colony stimulating factor 2 antibody, colony stimulating factor 2 (granulocyte-macrophage) antibody, CSF2 antibody, Csf2 antibody

Background: The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.Synonyms: CSF2, Colony stimulating factor 2, GMCSF, Granulocyte-macrophage colony-stimulating factor, Molgramostin, Sargramostim

Gene ID: 1437

UniProt: P04141

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin