Leptin antibody

Catalog No. ABIN181148

The Mouse Monoclonal anti-Leptin antibody is suitable to detect Leptin in samples from Human. It has been validated for WB and EIA.

Quick Overview for Leptin antibody (ABIN181148)

Target: Leptin (LEP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Leptin Antibody

Specificity: This antibody detects Leptin.

Characteristics: Synonyms: LEP, OB, OBS, Obesity factor, Obese protein

Purification: Affinity Chromatography on Protein A

Immunogen: Highly pure (>98%) recombinant Human Leptin

Isotype: IgG1

Application Details

Application Notes: ELISA: In a sandwich ELISA (assuming 100μL/well), a concentration of 2.0-4.0 μg/mL of thisantibody will detect recombinant human Leptin when used with Biotin conjugatedanti-Human Leptin antibody (cat. PP1041B) as the detection antibody at a concentration ofapproximately 0.5-1.0 μg/mL. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at aconcentration of 0.20-0.40 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant hLeptin is 2.0-4.0 ng/lane, undernon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS without preservatives

Preservative: Without preservative

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized antibody is stable at room temperature for one month or at -20 °C forlonger. Following reconstitution it is stable at 2-8 °C for six weeks. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype. Defects in the Leptin gene are the cause of profound obesity and type II diabetes.Synonyms: LEP, OB, OBS, Obese protein, Obesity factor

Gene ID: 3952

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process