MIA antibody (Biotin)

Catalog No. ABIN181669

Product Details anti-MIA Antibody (Biotin)

Target: MIA (Melanoma Inhibitory Activity (MIA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MIA antibody is conjugated to Biotin

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects MIA.

Purification: Affinity Chromatography.

Immunogen: Highly pure (> 98%) recombinant Human MIA

Application Details

Application Notes: Direct ELISA: To detect Human MIA (using 100 μL/well antibody solution) a concentration ofapprox. 1.0 μg/mL of this antibody is required. It allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human MIA. Sandwich ELISA: To detect Human MIA (using 100 μL/well antibody solution) aconcentration of 0.25-1.0 μg/mL of this antibody is required. In conjunction with PolyclonalAnti-Human MIA (ABIN181672 or ABIN181671) as a Capture antibody, it allows thedetection of at least 0.2-0.4 ng/well of recombinant Human MIA. Western blot: To detect Human MIA by Western Blot analysis this antibody can be used ata concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Human MIA is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2-8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.

Expiry Date: 6 months

Target Details for MIA

Target: MIA (Melanoma Inhibitory Activity (MIA))

Alternative Name: MIA (MIA Products)

Synonyms: CD-RAP antibody, Mia1 antibody, Cdrap antibody, zgc:194157 antibody, mia antibody, CDRAP antibody, Fdp antibody, MIA antibody, MIAL antibody, melanoma inhibitory activity antibody, otoraplin antibody, cilia and flagella associated protein 100 antibody, MIA antibody, Mia antibody, mia antibody, Otor antibody, CFAP100 antibody

Background: Melanoma Inhibitory Activity (MIA) was originally identified as an inhibitor of the in vitro growth of malignant melanoma cells. It was the first discovered member of a family of secreted cytokines termed the MIA/OTOR family. The four known members of this family, Melanoma Inhibitory Activity, MIA2, OTOR and TANGO each contain a Src homology-3 (SH3)-like domain. Melanoma Inhibitory Activity is an autocrine growth regulatory protein secreted from chondrocytes and malignant melanoma cells that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of Melanoma Inhibitory Activity may represent a clinically useful marker for diagnosis of melanoma metastasis as well as a potential marker for rheumatoid arthritis.Synonyms: Melanoma inhibitory activity, Melanoma-derived growth regulatory protein

Gene ID: 8190

NCBI Accession: NP_001189482

UniProt: Q16674