C10 / CCL6 antibody (Biotin)

Antigen: C10 / CCL6

Clonality: Polyclonal

Host: Rabbit

Reactivity: Mouse (Murine)

Conjugate: Biotin

Application: Enzyme Immunoassay (EIA), Western Blotting (WB)

Catalog no.: ABIN181759

Additional Information

Characteristics: Synonyms: C-C motif chemokine 6, Small-inducible cytokine A6, Protein C10, Scya6

Swiss-Prot: P27784

Immunogen: Highly pure (> 98 %) recombinant murine C10

Description: C10 belongs to the CC chemokine family and is expressed in myelopoietic bone marrowcultures when stimulated with GM-CSF, M-CSF, IL-3 or IL-4. It signals primarily through theCCR1 receptor. C10 is chemotactic for B cells, CD4+ T cells, monocytes and NK cells andalso exhibits powerful suppressive activity on colony formation by different lineages ofhematopoietic progenitors. The C10 contains the four highly conserved cysteine residuespresent in CC chemokines. The mature protein contains 95 amino acid residues.

Specificity: This antibody detects C-10. Species: Mouse. Others not tested.

Application Details

Application Notes: ELISA: Direct: To detect mC10 (using 100 μl/well antibody solution) a concentration of0.25 - 1.0 μg/ml of this antibody is required. In conjunction with compatible secondaryreagents, it allows the detection of at least 0.2 - 0.4 ng/well of recombinant mC10. Sandwich: To detect mC10 (using 100 μl/well antibody solution) a concentration of0.25 - 1.0 μg/ml of this antibody is required. In conjunction with Polyclonal Anti-Murine C10as a capture antibody, it allows the detection of at least 0.2 - 0.4 ng/well of recombinantmC10. Western blot: To detect mC10 this antibody can be used at a concentration of 0.1 - 0.2g/ml. Used in conjunction with compatible secondary reagents the detection limit forrecombinant mC-10 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Buffer: PBS, pH 7.2Label: BiotinReconstitution: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 %BSA to a concentration of 0.1 - 1.0 mg/ml.

Storage: Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeksat2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C. Avoid repeatedfreezing and thawing. Shelf life: One year from despatch.

Research Area: Complement Factors

Restrictions: For Research Use only