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CD8 antibody (FITC)

CD8 Reactivity: Guinea Pig FACS Host: Mouse Monoclonal CT6 FITC

Catalog No. ABIN182086

Datasheet as PDF

Target See all CD8 Antibodies

CD8
Reactivity
225

72

26

17

12

12

9

9

9

9

8

7

7

6

6

6

5

5

5

3

3

2

2
Guinea Pig
Host
229

53

39
Mouse
Compatible Secondaries
Clonality
265

47

2
Monoclonal
Conjugate
94

40

32

24

11

8

6

6

6

5

5

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CD8 antibody is conjugated to FITC
Application
271

65

64

46

44

42

29

19

17

14

14

14

8

5

4

3

1

1

1

1

1

1
Flow Cytometry (FACS)

Specificity

This anitbody reacts with an antigen present on a subset of T-lymphocyte with the same distribution as CD8 positive T-cells in man and Mouse.

Characteristics

Synonyms: T-lymphocyte differentiation antigen T8/Leu-2, T-cell surface glycoprotein CD8 alpha chain

Purification

Affinity Chromatography on Protein A

Immunogen

Guinea pig peritoneal T-cells. Spleen cells from immunised BALB/c mice were fused withcells of the X63. Ag8. 653 mouse myeloma cell line.

Clone

CT6

Isotype

IgG1

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anti-CD8 (CD8) (AA 51-150) antibody
CD8 Reactivity: Human, Mouse, Rat FACS, IHC (fro), WB, ELISA, IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PE

anti-CD8 (CD8) (AA 22-187) antibody
CD8 Reactivity: Human WB, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-CD8 (CD8) antibody
CD8 Reactivity: Human FACS, IP, CyTOF Host: Mouse Monoclonal MEM-31 unconjugated

anti-CD8 (CD8) antibody (PE)
CD8 Reactivity: Cat FACS Host: Mouse Monoclonal fCD8 PE

anti-CD8 (CD8) antibody
CD8 Reactivity: Human IHC, WB, IP, ELISA Host: Rabbit Monoclonal 3C3 unconjugated Recombinant Antibody

anti-CD8 (CD8) antibody (Biotin)
CD8 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-31 Biotin

anti-CD8 (CD8) antibody (FITC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 FITC

anti-CD8 (CD8) antibody (PerCP)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 PerCP

anti-CD8 (CD8) antibody (APC)
CD8 Reactivity: Human FACS Host: Mouse Monoclonal MEM-31 APC

Application Notes

Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions

For Research Use only
Concentration

0.1 mg/mL

Buffer

PBS, pH 7.4, 0.09 % Sodium Azide, 1 % BSA

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date

12 months
Target

CD8

Alternative Name

CD8 (CD8 Products)

Synonyms

CD8 antibody, Leu2 antibody, MAL antibody, p32 antibody, CD8a molecule antibody, CD8A antibody

Background

The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell to cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T cell receptor on the T lymphocyte recognize antigen displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.Synonyms: CD8 alpha chain, CD8A, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2

Gene ID

925

UniProt

P01732