Transcriptional Adaptor 3 (TADA3) (Middle Region) antibody

Details for Product No. ABIN182375
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Antigen
Synonyms ada3, ada3-B, ngg1, tada3l, tada3l-B, TADA3L, ADA3, NGG1, STAF54, hADA3, 1110004B19Rik, AI987856, Tada3l, fa03f04, tada3, wu:fa03f04, zgc:63596
Epitope
Middle Region
(11), (8), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Cow (Bovine), Dog (Canine), Xenopus laevis, Zebrafish (Danio rerio), Human, Chicken
(37), (6), (5), (3), (3), (3), (3), (3), (1)
Host
Rabbit
(30), (7)
Clonality
Polyclonal
Conjugate
Un-conjugated
(3), (3), (3), (1), (1), (1)
Application
Western Blotting (WB)
(37), (21), (7), (3), (3), (3), (1)
Pubmed 2 references available
Quantity 50 μg
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Catalog No. ABIN182375
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Immunogen Synthetic peptide directed towards the middle region of human TADA3L
Sequence ASVEPYCADI TSEEVRTLEE LLKPPEDEAE HYKIPPLGKH YSQRWAQEDL
Predicted Reactivity Zebrafish : 78 %
Characteristics This is a rabbit polyclonal antibody against TADA3L. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Alternative Name TADA3L
Background Adaptor proteins are usually required for transcriptional activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter.TADA3L is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis.Many DNA-binding transcriptional activator proteins enhance the initiation rate of RNA polymerase II-mediated gene transcription by interacting functionally with the general transcription machinery bound at the basal promoter. Adaptor proteins are usually required for this activation, possibly to acetylate and destabilize nucleosomes, thereby relieving chromatin constraints at the promoter. The protein encoded by this gene is a transcriptional activator adaptor and has been found to be part of the PCAF histone acetylase complex. In addition, it associates with the tumor suppressor protein p53 and is required for full activity of p53 and p53-mediated apoptosis. At least four alternatively spliced variants have been found for this gene, but the full-length nature of some variants has not been determined.
Molecular Weight 49 kDa
Gene ID 10474
NCBI Accession NM_006354, NP_006345
UniProt O75528
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 432 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Transcriptional Adaptor 3 (TADA3) (Middle Region) antibody WB Suggested Anti-TADA3L Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:12500
Positive Control: Jurkat cell lysate
anti-Transcriptional Adaptor 3 (TADA3) (Middle Region) antibody (2) WB Suggested Anti-TADA3L
Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500
Positive Control: Jurkat cell lysate
Background publications Rual, Venkatesan, Hao et al.: "Towards a proteome-scale map of the human protein-protein interaction network." in: Nature, Vol. 437, Issue 7062, pp. 1173-8, 2005 (PubMed).

General Shamanin, Sekaric, Androphy: "hAda3 degradation by papillomavirus type 16 E6 correlates with abrogation of the p14ARF-p53 pathway and efficient immortalization of human mammary epithelial cells." in: Journal of virology, Vol. 82, Issue 8, pp. 3912-20, 2008 (PubMed).

Validation Images
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