ERCC8 antibody (N-Term)

Catalog No. ABIN182497

This Rabbit Polyclonal antibody specifically detects ERCC8 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Guinea Pig and Zebrafish (Danio rerio).

Quick Overview for ERCC8 antibody (N-Term) (ABIN182497)

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Reactivity: Human, Mouse, Rat, Dog, Rabbit, Cow, Pig, Guinea Pig, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ERCC8 Antibody

Binding Specificity: N-Term

Sequence: DVERIHGGGI NTLDIEPVEG RYMLSGGSDG VIVLYDLENS SRQSYYTCKA

Predicted Reactivity: Cow: 77%, Dog: 85%, Guinea Pig: 77%, Human: 100%, Mouse: 85%, Pig: 85%, Rabbit: 85%, Rat: 77%, Zebrafish: 85%

Characteristics: This is a rabbit polyclonal antibody against ERCC8. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ERCC8

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 396 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Target Details for ERCC8

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: ERCC8

Background: ERCC8 is a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes.
Alias Symbols: CKN1, CSA
Protein Interaction Partner: CUL4A, CUL4B, DDB1, RUVBL2, ZNF24, PCNA, H1F0, ERCC6, UVSSA, DDA1, RFWD2, COPS4, COPS6, COPS3, TOP1, TP53, SSBP1, OGG1, MDM2, GTF2H2, CUL5, GPS1, DDB2, COPS2, COPS7A, COPS5, COPS8, RBX1, POLR2A, HMGN1, EP300, UBC, CSNK2B, XAB2, UQCRQ, CBR1,
Protein Size: 396

Molecular Weight: 44 kDa

Gene ID: 1161

NCBI Accession: NM_000082, NP_000073

UniProt: Q13216

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus