ERCC8 antibody (AA 161-260)

Catalog No. ABIN1713778

This Rabbit Polyclonal antibody specifically detects ERCC8 in WB, ELISA, IF (cc), IF (p), IHC (p), ICC and IHC (fro). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERCC8 antibody (AA 161-260) (ABIN1713778)

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-ERCC8 Antibody

Binding Specificity: AA 161-260

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Horse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ERCC8/CSA

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for ERCC8

Target: ERCC8 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 8 (ERCC8))

Alternative Name: ERCC8/CSA

Background:

Synonyms: CSA, CKN1, UVSS2, DNA excision repair protein ERCC-8, Cockayne syndrome WD repeat protein CSA, ERCC8

Background: Substrate-recognition component of the CSA complex, a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, involved in transcription-coupled nucleotide excision repair. The CSA complex (DCX(ERCC8) complex) promotes the ubiquitination and subsequent proteasomal degradation of ERCC6 in a UV-dependent manner, ERCC6 degradation is essential for the recovery of RNA synthesis after transcription-coupled repair. It is required for the recruitment of XAB2, HMGN1 and TCEA1/TFIIS to a transcription-coupled repair complex which removes RNA polymerase II-blocking lesions from the transcribed strand of active genes.

Gene ID: 1161

UniProt: Q13216

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus