Zinc Finger Protein 282 (ZNF282) (C-Term) antibody

Details for Product No. ABIN183643
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Antigen
Synonyms HUB1, Znf282
Epitope
C-Term
(2), (1), (1)
Reactivity
Mouse (Murine), Rat (Rattus), Dog (Canine), Zebrafish (Danio rerio), Cow (Bovine), Human
(5), (1), (1), (1), (1)
Host
Rabbit
(5)
Clonality
Polyclonal
Application
Western Blotting (WB)
(5)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN183643
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Immunogen Synthetic peptide directed towards the C terminal of human ZNF282
Sequence FIRKQNLLKH QRIHTGERPY TCGECGKSFR YKESLKDHLR VHSGGPGPGA
Predicted Reactivity Bovine : 100 %, Dog : 100 %, Human : 100 %, Rabbit : 100 %
Characteristics This is a rabbit polyclonal antibody against ZNF282. It was validated on Western Blot using a cell lysate as a positive control.
Purification Protein A purified
Alternative Name ZNF282
Background ZNF282 contains 1 KRAB domain and 5 C2H2-type zinc fingers. It belongs to the krueppel C2H2-type zinc-finger protein family and binds to the U5 repressive element (U5RE) of the human T cell leukemia virus type I long terminal repeat.
Molecular Weight 74 kDa
Gene ID 8427
NCBI Accession NM_003575, NP_003566
UniProt Q9UDV7
Research Area Chromatin and Nuclear Signaling, Transcription Factors
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 671 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Zinc Finger Protein 282 (ZNF282) (C-Term) antibody Lanes: Lane 1:241 µg Bewo cells Lane 2: 041 µg HEK cells Lane 3: 041 µg JEG3 cells Lane 4: 041 µg PC3 cells Lane 5: 041 µg SHEP cells
Primary Antibody Dilution: 1:0000
Secondary Antibody: Anti-rabbit-HRP
Secondary Antibody Dilution: 1:0500
Gene Name: ZNF282

Submitted by: Lisa Stubbs, University of Illinois
Background publications Cheng, Kapranov, Drenkow et al.: "Transcriptional maps of 10 human chromosomes at 5-nucleotide resolution." in: Science (New York, N.Y.), Vol. 308, Issue 5725, pp. 1149-54, 2005 (PubMed).

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