SNRNP70 antibody (N-Term)

Catalog No. ABIN183913

This Rabbit Polyclonal antibody specifically detects SNRNP70 in WB and IHC. It exhibits reactivity toward Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Rabbit and Horse and has been mentioned in 2+ publications.

Quick Overview for SNRNP70 antibody (N-Term) (ABIN183913)

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Reactivity: Human, Mouse, Rat, Dog, Zebrafish (Danio rerio), Cow, Guinea Pig, Rabbit, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRNP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SNRNP70 Antibody

Binding Specificity: N-Term

Sequence: PHNDPNAQGD AFKTLFVARV NYDTTESKLR REFEVYGPIK RIHMVYSKRS

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 100%

Characteristics: This is a rabbit polyclonal antibody against SNRP70. It was validated on Western Blot and immunohistochemistry.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human SNRP70

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 437 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-SNRNP70 antibody (ABIN183913)

Wang, Mueller, Hertel, Cambi: "G Run-mediated recognition of proteolipid protein and DM20 5' splice sites by U1 small nuclear RNA is regulated by context and proximity to the splice site." in: The Journal of biological chemistry, Vol. 286, Issue 6, pp. 4059-71, (2011) (PubMed).

Wang, Cambi: "Heterogeneous nuclear ribonucleoproteins H and F regulate the proteolipid protein/DM20 ratio by recruiting U1 small nuclear ribonucleoprotein through a complex array of G runs." in: The Journal of biological chemistry, Vol. 284, Issue 17, pp. 11194-204, (2009) (PubMed).

Target Details for SNRNP70

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Alternative Name: SNRP70

Background: SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.
Alias Symbols: RPU1, Snp1, U1AP, U170K, U1RNP, RNPU1Z, SNRP70, U1-70K
Protein Interaction Partner: IL32, UBC, NEDD8, LIN28A, EZH2, SUZ12, EED, RNF2, rev, WBP4, SRPK2, SRPK1, MYC, SRPK3, HDAC2, PAN2, SMURF2, PRPF40A, CDKL3, RNF11, PRKD2, SKIL, RELA, RASGRF1, SMAD9, SMAD5, SMAD4, SMAD3, SMAD2, CHERP, PRMT5, UBL4A, VCAM1, SMAD1, ITGA4, IL7R, IFIT3, IFIT1,
Protein Size: 437

Molecular Weight: 52 kDa

Gene ID: 6625

NCBI Accession: NM_003089, NP_003080

UniProt: P08621