SNRNP70 antibody (C-Term)

Catalog No. ABIN183955

This Rabbit Polyclonal antibody specifically detects SNRNP70 in WB and IHC. It exhibits reactivity toward Human, Rat, Mouse, Dog, Cow, Guinea Pig and Rabbit. It has been mentioned in 3+ publications

Quick Overview for SNRNP70 antibody (C-Term) (ABIN183955)

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Reactivity: Human, Rat, Mouse, Dog, Cow, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRNP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-SNRNP70 Antibody

Binding Specificity: C-Term

Purpose: SNRP70 Antibody - C-terminal region

Sequence: RERERKEELR GGGGDMAEPS EAGDAPPDDG PPGELGPDGP DGPEEKGRDR

Predicted Reactivity: Cow: 100%, Dog: 100%, Guinea Pig: 100%, Human: 100%, Mouse: 93%, Rabbit: 100%, Rat: 100%

Characteristics: This is a rabbit polyclonal antibody against SNRP70. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human SNRP70

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8111922

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-SNRNP70 antibody (ABIN183955)

Franklin, Chen, Mitchell-Jordan, Ren, Wang, Vondriska: "Quantitative analysis of the chromatin proteome in disease reveals remodeling principles and identifies high mobility group protein B2 as a regulator of hypertrophic growth." in: Molecular & cellular proteomics : MCP, Vol. 11, Issue 6, pp. M111.014258, (2012) (PubMed).

Serini, Fasano, Piccioni, Monego, Cittadini, Celleno, Ranelletti, Calviello: "DHA induces apoptosis and differentiation in human melanoma cells in vitro: involvement of HuR-mediated COX-2 mRNA stabilization and ?-catenin nuclear translocation." in: Carcinogenesis, Vol. 33, Issue 1, pp. 164-73, (2011) (PubMed).

Franklin, Zhang, Chen, Paulsson, Mitchell-Jordan, Li, Ping, Vondriska: "Specialized compartments of cardiac nuclei exhibit distinct proteomic anatomy." in: Molecular & cellular proteomics : MCP, Vol. 10, Issue 1, pp. M110.000703, (2011) (PubMed).

Target Details for SNRNP70

Target: SNRNP70 (Small Nuclear Ribonucleoprotein 70kDa (U1) (SNRNP70))

Alternative Name: SNRP70

Background:

Background Information: SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA. Western blots using two different antibodies against two unique regions of this protein target confirm the same apparent molecular weight in our tests.

Gene Name: Small nuclear ribonucleoprotein 70 kDa (U1)

Alternative Symbols: RPU1, Snp1, U1AP, U170K, U1RNP, RNPU1Z, SNRP70, U1-70K

Protein Name: U1 small nuclear ribonucleoprotein 70 kDa

Molecular Weight: 52 kDa

Gene ID: 6625

NCBI Accession: NP_003080

UniProt: P08621