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Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody

Details for Product No. ABIN184021
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Antigen
Synonyms HNRPUL1, E1B-AP5, E1BAP5, E130317O14Rik, Hnrnpul, Hnrpul1, hnrpul1, wu:fb53d10, wu:fk45c03, zgc:85971
Epitope
C-Term
(5), (3), (2), (1), (1), (1)
Reactivity
Dog (Canine), Mouse (Murine), Rat (Rattus), Cow (Bovine), Human
(18), (5), (4), (1), (1), (1)
Host
Rabbit
(20), (1)
Clonality
Polyclonal
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC)
(20), (7), (7), (4), (4), (2), (2)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184021
229.00 $
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Immunogen Synthetic peptide directed towards the C terminal of human HNRPUL1
Sequence TYPQPSYNQYQQYAQQWNQYYQNQGQWPPYYGNYDYGSYS GNTQGGTSTQ
Predicted Reactivity Bovine :100 %,   White-tufted-ear marmoset :100 %,   Dog :100 %,   Human :100 %,   Rat :80 %,   Mouse :80 %,  
Characteristics This is a rabbit polyclonal antibody against HNRPUL1. It was validated on Western Blot and immunohistochemistry.
Purification Protein A purified
Alternative Name HNRPUL1
Background HNRPUL1 is a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein binds specifically to adenovirus E1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus-infected cells.This gene encodes a nuclear RNA-binding protein of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. This protein binds specifically to adenovirus E1B-55kDa oncoprotein. It may play an important role in nucleocytoplasmic RNA transport, and its function is modulated by E1B-55kDa in adenovirus-infected cells. Four transcript variants encoding different isoforms have been found for this gene. Another variant has also been found, but its full-length nature has not been determined.
Molecular Weight 83 kDa
Gene ID 11100
NCBI Accession NM_144732, NP_653333
UniProt Q9BUJ2-4
Research Area Chromatin and Nuclear Signaling, Chromatin Binding Proteins, Transcription Factors, DNA/RNA
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 756 AA

Restrictions For Research Use only
Format Lyophilized
Reconstitution Add 100 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For longer periods of storage, store at -20 °C
Supplier Images
anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody WB Suggested Anti-HNRPUL1 Antibody Titration: 0.25ug/ml
ELISA Titer: 1:312500
Positive Control: Raji cell lysate
anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (2) anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (Image 2)
anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (3) anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (Image 3)
anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (4) Amount and Sample Type: Lane 1:5 % Input, Lane 2: %% Sup, Lane 3: Normal IgG, Lane 4: hn-RNPUL1 ppt. k562 sample
IP Antibody: HNRPUL1
Amount of
IP Antibody:
Primary Antibody: HNRPUL1
Primary Antibody Dilution: 1: 1000
Secondary Antibody: Anti-rabbit-HRP
Secondary Antibody Dilution: 1: 1000
Gene Name: HNRPUL1

Submitted by: Anonymous
anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (5) Lanes: Lane 1:41 µg HeLa S3 Lane 2: 141 µg HeLa S3
Primary Antibody Dilution: 1: u000
Secondary Antibody: Anti-rabbit-HRP
Secondary Antibody Dilution: 1: u000
Gene Name: HNRPUL1

Submitted by: Anonymous
anti-Heterogeneous Nuclear Ribonucleoprotein U-Like 1 (HNRNPUL1) (C-Term) antibody (6) Sample Type: MCF7 cells
Primary Antibody Dilution: 1:200
Secondary Antibody: Anti-rabbit-FITC
Secondary Antibody Dilution: 1:000 Color/Signal Descriptions: DAPI: Blue HNRNPUL1: Green
Gene Name: HNRPUL1

Submitted by: Anonymous
Background publications Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Validation Images
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