»
Antibodies
»
anti-Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) antibody
Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B) antibody
| Antigen |
|
| Synonyms |
CDC25B, AI604853 |
| Clonality |
Polyclonal |
|
Host
|
|
|
Reactivity
|
 Alternatives Cow (Bovine), Human, Dog (Canine), Mouse (Murine), Rat (Rattus), Pig (Porcine)
|
|
Conjugate
|
Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (4), Biotin (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), Alexa Flour 488 (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
|
|
Application
|
Alternatives Western Blotting (WB), Immunohistochemistry (IHC)
|
| Catalog no. |
ABIN184115 |
| Quantity |
50 µg (Variants) |
| Price |
317.90 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 2 to 3 Business Days |
|
Alternative name
|
CDC25B
|
|
Gene ID
|
CDC25B
|
|
Immunogen
|
The immunogen for anti-CDC25B antibody: synthetic peptide directed towards the N terminal of human CDC25B
|
|
Sequence
|
TQTMHDLAGLGSETPKSQVGTLLFRSRSRLTHLSLSRRAS ESSLSSESSE
|
|
Cross-Reactivity
|
Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus)
|
|
Format
|
Lyophilized
|
|
Reconstitution
|
Add 50 µl of distilled water. Final anti-CDC25B antibody concentration is 1 mg/ml.
|
|
Description
|
CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined.CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.
|
|
Characteristics
|
Antigen Size: 580 amino acids
|
|
Molecular Weight
|
65kDa
|
|
Application Notes
|
WB Suggested Anti-CDC25B Antibody Titration: 0.2-1 ug/ml
Positive Control: HepG2 cell lysate.
|
|
Purification
|
Affinity Purified
|
|
Buffer
|
PBS
|
|
Storage (Long Term)
|
For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
|
|
Storage (Short Term)
|
Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.
|
|
Research Area
|
Cell Cycle, Cancer, Kinases/Phosphatases
|
|
Restrictions
|
For Research Use only
|
|
Product
|
Mirey, Chartrain, Froment et al.: "CDC25B phosphorylated by pEg3 localizes to the centrosome and the spindle poles at mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 4, Issue 6, pp. 806-11, 2005 (PubMed).
|
Alternatives for antigen "Cell Division Cycle 25 Homolog B (S. Pombe) (CDC25B)", type "Antibodies"
|
Hosts
|
Rabbit (99), Mouse (5), Chicken (1)
|
|
Reactivities
|
Human (98), Mouse (Murine) (62), Rat (Rattus) (62), Dog (Canine) (21), Cow (Bovine) (20), Pig (Porcine) (20), Cat (Feline) (1), Chicken (1)
|
|
Applications
|
Immunofluorescence (IF) (54), Western Blotting (WB) (54), ELISA (42), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (21), Immunohistochemistry (IHC) (19), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoprecipitation (IP) (5), Immunocytochemistry (ICC) (2), Immunoelectron Microscopy (IEM) (2)
|
|
Conjugates
|
Alexa Fluor 350 (5), Alexa Fluor 488 (5), Alexa Fluor 555 (5), Alexa Fluor 647 (5), PE,Cy5.5 (4), Biotin (3), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), Alexa Flour 488 (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
|
|
Epitopes
|
Ser149 (12), pSer149 (7), pSer186 (7), pSer353 (7), pSer323 (6), N-Term (4), Internal Region (3), Ser353 (2), pSer187 (2), AA 140-185 (1), C-Term (1), Center (1), N-Term, Ala166 (1), pSer124 (1)
|
2013 BioMed Central Research Awards
