NLR Family, CARD Domain Containing 4 (NLRC4) antibody

Antigen: NLR Family, CARD Domain Containing 4 (NLRC4)

Synonyms: CLAN, IPAF, CLAN1, CLANA, CLANB, CLANC, CLAND, CARD12, CLR2.1, NLRC4

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184136

Additional Information

Alternative name: NLRC4

Gene ID: NLRC4

UniProt: Q9NPP4

Immunogen: The immunogen for anti-NLRC4 antibody: synthetic peptide directed towards the C terminal of human NLRC4

Sequence: QLNLAGNRVSSDGWLAFMGVFENLKQLVFFDFSTKEFLPD PALVRKLSQV

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Format: Lyophilized

Reconstitution: Add 50 µl of distilled water. Final anti-NLRC4 antibody concentration is 1 mg/ml.

Description: In C. elegans, Ced4 binds and activates Ced3, an apoptotic initiator caspase, via caspase-associated recruitment domains (CARDs). Human Ced4 homologs include APAF1, NOD1, and NOD2. These proteins have at least 1 N-terminal CARD domain followed by a centrally located nucleotide-binding domain (NBD or NACHT) and a C-terminal regulatory domain, found only in mammals, that contains either WD40 repeats or leucine-rich repeats (LRRs). CARD12 is a member of the Ced4 family and can induce apoptosis.

Characteristics: Antigen Size: 1024 amino acids

Specificity: This antibody will recognize NLRC4 isoform 1 (116kD) and isoform 2 (40kD).

Molecular Weight: 116kDa

Application Details

Application Notes: WB Suggested Anti-NLRC4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate.

Purification: Affinity Purified

Buffer: PBS

Storage (Long Term): For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.

Storage (Short Term): Any unfrozen and/or unused material can be stored at 4°C for short term use (< 1 week), and should not be re-frozen.

Restrictions: For Research Use only

Images

WB Suggested Anti-NLRC4 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: 1:62500
Positive Control: HepG2 cell lysate

anti-NLR Family, CARD Domain Containing 4 (NLRC4) antibody (Image 2)

Publications

Product: Clark, Gurney, Abaya et al.: "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." in: Genome research, Vol. 13, Issue 10, pp. 2265-70, 2003 (PubMed).