Rabbit Anti-Stathmin 1 (Phospho-Ser16) Polyclonal Antibody detects endogenous levels of Stathmin 1 only when phosphorylated at serine 16.
Purification
Rabbit Anti-Stathmin 1 (Phospho-Ser16) Polyclonal Antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Immunogen
Synthesized phosphopeptide derived from human Stathmin 1 around pSer16 (R-A-SP-G-Q).
STMN1
Reactivity: Human
WB, IHC, ELISA
Host: Rabbit
Polyclonal
unconjugated
Application Notes
IHC: 1:50-1:100
Restrictions
For Research Use only
Concentration
1 mg/mL
Buffer
phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice
Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Avoid repeated freezing and thawing.
Storage
-20 °C
Storage Comment
Store at -20 °C for 1 year.
Lin, Li, Shan, Wang, Saito, Xu, Di, Zhong, Li: "Reduced stathmin-1 expression in natural killer cells associated with spontaneous abortion." in: The American journal of pathology, Vol. 178, Issue 2, pp. 506-14, (2011) (PubMed).