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DDB1 antibody (Damage Specific DNA Binding Protein 1) (C-Term)

Details for Product anti-DDB1 Antibody No. ABIN184570, Supplier: Log in to see
Antigen
  • 127kDa
  • AA408517
  • DDBA
  • p127-Ddb1
  • UV-DDB1
  • XAP1
  • XPCE
  • XPE
  • XPE-BF
Epitope
C-Term
27
7
6
3
2
2
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
88
34
28
8
6
4
4
3
2
2
2
2
1
1
1
Host
Goat
49
22
18
Clonality
Polyclonal
Conjugate
This DDB1 antibody is un-conjugated
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
82
38
28
24
16
13
7
4
2
1
1
1
1
1
Supplier
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Immunogen Peptide with sequence C-DLIKVVEELTRIH, from the C Terminus of the protein sequence according to NP_001914.3.
Blocking Peptide Blocking peptide for this product available: ABIN368888
Cross-Reactivity Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name DDB1 (DDB1 Antibody Abstract)
Background Synonyms: DDB1, damage-specific DNA binding protein 1, 127kDa, DDBA, XAP1, XPCE, XPE-BF, UV-DDB1, DDB p127 subunit, damage-specific DNA binding protein 1 (127kD), XPE
Gene ID 1642, 64470
NCBI Accession NP_001914
Research Area Chromatin and Nuclear Signaling, DNA/RNA
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 130 kDa band seen in HeLa, Jurkat and NSO (mouse) cell lysates. Recommended for use at 0.5-1.5 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-DDB1 antibody (Damage Specific DNA Binding Protein 1) (C-Term) (ABIN184570) ABIN184570 (1 µg/ml) staining of NSO lysate (1E5 cells per lane). Detected by western...
Product cited in: Li, Robert, van Breugel, Strubin, Zheng: "A promiscuous alpha-helical motif anchors viral hijackers and substrate receptors to the CUL4-DDB1 ubiquitin ligase machinery." in: Nature structural & molecular biology, Vol. 17, Issue 1, pp. 105-11, 2010 (PubMed).

Background publications Dualan, Brody, Keeney, Nichols, Admon, Linn: "Chromosomal localization and cDNA cloning of the genes (DDB1 and DDB2) for the p127 and p48 subunits of a human damage-specific DNA binding protein." in: Genomics, Vol. 29, Issue 1, pp. 62-9, 1996 (PubMed).