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Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A) antibody

Antigen

Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A)

Synonyms GADD34, Peg-3, Myd116, PPP1R15A, MGC137534
Clonality Polyclonal
Host
Reactivity
Alternatives

Human

Application
Alternatives ELISA, Western Blotting (WB)
1 reference available
Catalog no. ABIN184571
Quantity 100 ug  (Variants)
Price 501.80 $   Plus shipping costs $35.00
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Additional Information

Alternative name PPP1R15A / GADD34
Gene ID 23645
Immunogen C-AAALDLSGRRG
Format Affinity-purified
Description All names and symbols: PPP1R15A, GADD34, protein phosphatase 1, regulatory (inhibitor) subunit 15A, growth arrest and DNA-damage-inducible 34, protein phosphatase 1, regulatory subunit 15A. Official Symbol: PPP1R15A. RefSeq number(s): NP_055145.2. Gene ontology terms: cell cycle arrest, response to DNA damage stimulus, apoptosis
Specificity Human

Application Details

Application Notes Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 75kDa band observed in lysates of cell line HEPG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.1-0.3µg/ml.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Storage Aliquot and store at -20°C. Minimize freezing and thawing.
Restrictions For Research Use only

Publications

Publications Kojima, Takeuchi, Haneda et al.: "The function of GADD34 is a recovery from a shutoff of protein synthesis induced by ER stress: elucidation by GADD34-deficient mice." in: The FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 17, Issue 11, pp. 1573-5, 2003 (PubMed).

Alternatives

Alternatives for antigen "Protein Phosphatase 1, Regulatory (Inhibitor) Subunit 15A (PPP1R15A)", type "Antibodies"
Hosts Rabbit (20), Goat (7)
Reactivities Human (26), Mouse (Murine) (20), Rat (Rattus) (20), Cow (Bovine) (3), Chicken (2), Sheep (Ovine) (2), Dog (Canine) (1), Horse (Equine) (1), Pig (Porcine) (1), Rabbit (1)
Applications Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (9), ELISA (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (2), Immunoprecipitation (IP) (2), Enzyme Immunoassay (EIA) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
Epitopes C-Term (2)