Aurora Kinase A (AURKA) antibody

Antigen: Aurora Kinase A (AURKA)

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184572

Additional Information

Alternative name: Aurora kinase A / AURKA

Gene ID: 6790

Immunogen: C-QNKESASKQS

Format: Affinity-purified

Description: All names and symbols: AURKA, aurora kinase A, AIK, ARK1, AURA, AURORA2, BTAK, MGC34538, STK15, STK6, STK7, IPL1-related kinase, aurora-A, aurora-related kinase 1, aurora/IPL1-like kinase, breast-tumor-amplified kinase, serine/threonine kinase 15, serine/threonine kinase 6, serine/threonine protein kinase 15, serine/threonine protein kinase 6. Official Symbol: AURKA. RefSeq number(s): NP_003591.2. Gene ontology terms: cAMP-dependent protein kinase activity, protein kinase CK2 activity, protein tyrosine kinase activity, ATP binding, protein serine/threonine kinase activity, cell cycle, mitosis, protein amino acid phosphorylation, oncogenesis, spindle, spindle pole body, nucleus, transferase activity

Specificity: Human

Synonyms: AIK, ARK1, AURA, BTAK, STK6, STK7, STK15, AURORA2, MGC34538, IAK, Ark1, Ayk1, Stk6, AIRK1, AU019385, AW539821, Aurora-A, 87A7-9/2, AurA, ck10, ck[10], l(3)87Ac, l(3)ck10, DmelCG3068, CG3068, MGC124935, aurka, AURKA, MGC134472, aik, ark1, aura, btak, stk6, stk7, stk15, aurora2, zgc:100912

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:128,000. Western Blot: Approx 45kDa band observed in lysates of cell line HeLa and Jurkat lysate (calculated MW of 45.8kDa according to NP_003591.2). In transfected HEK293 transiently expressing AURKA a band of approx. 48kDa is observed. This band is not observed in the non-transfected HEK293.Recommended concentration: 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184572 (0.1µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

HEK293 overexpressing Aurora kinase A (RC212018) with C-terminal tag (DYKDDDDK) and probed with anti-DYKDDDDK in the left panel and with ABIN184572 in the right panel (vector-only transfection in first and fourth lanes).

Publications

Publications: Zhou, Kuang, Zhong et al.: "Tumour amplified kinase STK15/BTAK induces centrosome amplification, aneuploidy and transformation." in: Nature genetics, Vol. 20, Issue 2, pp. 189-93, 1998 (PubMed).