Suppressor of Cytokine Signaling 1 (SOCS1) antibody

Antigen: Suppressor of Cytokine Signaling 1 (SOCS1)

Synonyms: JAB, CIS1, SSI1, TIP3, CISH1, SSI-1, SOCS-1, Cish1, Socs-1, zgc:91868, SOCS1, jab, cis1, ssi1, tip3, cish1, ssi-1, socs-1, socs1, LOC100228306, socs1b

Clonality: Polyclonal

Host: Goat

Reactivity: Mouse (Murine), Human

Application: ELISA, Western Blotting (WB)

Catalog no.: ABIN184575

Additional Information

Alternative name: SOCS1

Gene ID: 8651, 12703 (mouse)

Immunogen: C-VLRDYLSSFPFQI

Format: Affinity-purified

Description: All names and symbols: SOCS1, JAB, CIS1, SSI1, TIP3, CISH1, Cish1, SSI-1, SOCS-1, suppressor of cytokine signaling 1, JAK binding protein, Tec-interacting protein 3, STAT induced SH3 protein 1, cytokine-inducible SH2 protein 1. Official Symbol: SOCS1. RefSeq number(s): NP_003736.1. Gene ontology terms: protein kinase inhibitor activity, JAK-STAT cascade, intracellular signaling cascade, cytoplasm, regulation of cell growth

Specificity: Human, Mouse, Rat

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:4,000. Western Blot: In transfected HEK293 transiently expressing human SOCS1 bands of approx. 30kDa and 25kDa are observed. These bands are not observed in the non-transfected HEK293. A similar pattern is observed in Mouse Spleen lysates. The calculated molecular size is 23.6kDa according to Human NP_003736.1 and 23.7kDa according to Mouse NP_034026.1. Recommended concentration, 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Chromatin and Nuclear Signaling, Signaling, Cytokines

Restrictions: For Research Use only

Images

ABIN184575 staining (2µg/ml) of mouse spleen lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

HEK293 overexpressing SOCS1 and probed with ABIN184575 (mock transfection in lane B).

Publications

Publications: Starr, Willson, Viney et al.: "A family of cytokine-inducible inhibitors of signalling." in: Nature, Vol. 387, Issue 6636, pp. 917-21, 1997 (PubMed).