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Neutrophil Cytosolic Factor 1 (NCF1) antibody
| Antigen | Neutrophil Cytosolic Factor 1 (NCF1) |
| Synonyms | NCF1A, NOXO2, p47phox, FLJ79451, SH3PXD1A |
| Clonality | Polyclonal |
| Host |
 Alternatives Goat |
| Reactivity |
Alternatives Human |
| Application |
Alternatives ELISA, Western Blotting (WB)
|
 |
1 reference available |
| Catalog no. | ABIN184583 |
| Quantity | 100 ug (Variants) |
| Price | 501.80 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 5 Business Days |
Additional Information
| Alternative name | NCF1 / p47phox |
| Gene ID | 653361, 17969 (mouse), 114553 (rat) |
| Immunogen | C-SESTKRKLASAV |
| Format | Affinity-purified |
| Description | All names and symbols: NCF1, p47phox, FLJ79451, NCF1A, NOXO2, SH3PXD1A, neutrophil cytosolic factor 1, Neutrophil cytosolic factor-1 (47kD), neutrophil cytosolic factor 1 (47kD, chronic granulomatous disease, autosomal 1), NADPH oxidase organizer 2. Official Symbol: NCF1. RefSeq number(s): NP_000256.3. Gene ontology terms: GTPase activity, protein binding, electron transporter activity, electron transport, superoxide metabolism, cellular defense response, intracellular signaling cascade, cytosol, soluble fraction |
| Specificity | Human, Mouse, Rat, Rabbit, Cow |
Application Details
| Application Notes | Western blot: Approx 47kDa band seen in U937 cell lysates. Recommended for use at 0.2-2µg/ml. |
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Restrictions | For Research Use only |
Images
| ABIN184583 staining (0.2µg/ml) of U937 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. |
Publications
| Publications |
Davidson-Moncada, Lopez-Lluch, Segal et al.: "Involvement of protein kinase D in Fc gamma-receptor activation of the NADPH oxidase in neutrophils." in: The Biochemical journal, Vol. 363, Issue Pt 1, pp. 95-103, 2002 (PubMed).
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Alternatives
Alternatives for antigen "Neutrophil Cytosolic Factor 1 (NCF1)", type "Antibodies"
| Hosts | Rabbit (14), Goat (2) |
| Reactivities | Human (14), Mouse (Murine) (5), Rat (Rattus) (5), Cow (Bovine) (1), Dolphin (1), Rabbit (1) |
| Applications | Western Blotting (WB) (13), ELISA (10), Immunohistochemistry (IHC) (10), Immunofluorescence (IF) (3), Immunocytochemistry (ICC) (1), Immunoprecipitation (IP) (1) |
| Epitopes | Ab-359 (1), pSer304 (1), pSer328 (1), pSer345 (1), pSer359 (1), pSer370 (1) |
