Protein Phosphatase 2, Regulatory Subunit B', epsilon Isoform (PPP2R5E) (C-Term) antibody

Details for Product No. ABIN184647
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Antigen
Synonyms wdb2, ppp2r5e, ppp2r5e1, PPP2R5E, 4633401M22Rik, AI449017, B56beta
Epitope
C-Term
(4), (3), (2), (1), (1)
Reactivity
Mouse (Murine)
(12), (4), (2), (1), (1)
Host
Goat
(8), (6), (1)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(13), (3), (1), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184647
374.27 $
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Immunogen Peptide with sequence C-LKRGLRRDGIIPT, from the C Terminus of the protein sequence according to NP_006237.1.
Cross-Reactivity Human, Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name PPP2R5E
Background Synonyms: PPP2R5E, protein phosphatase 2, regulatory subunit B (B56), epsilon isoform, PP2A, B subunit, B￵psilon isoform, PP2A, B subunit, R5 epsilon isoform, PP2A, B subunit, B56 epsilon isoform, PP2A, B subunit, PR61 epsilon isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon isoform, protein phosphatase 2, regulatory subunit Bepsilon isoform, PP2A, B subunit, R5 epsilon, epsilon isoform of regulatory subunit B56, protein phosphatase 2A, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon
Gene ID 5529
NCBI Accession NP_006237
Research Area Signaling
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 55 kDa and 40 kDa bands seen in mouse brain extracts. Recommended for use at 0.5-2 µg/mL. Please note that the two bands may correspond to two different isoforms. Two isoforms of the epsilon mRNA have been detected in Northern blots (J Biol Chem 1996 Sep 6,271(36):22081-9).
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Protein Phosphatase 2, Regulatory Subunit B', epsilon Isoform (PPP2R5E) (C-Term) antibody ABIN184647 staining (0.5µg/ml) of mouse brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General McCright, Virshup: "Identification of a new family of protein phosphatase 2A regulatory subunits." in: The Journal of biological chemistry, Vol. 270, Issue 44, pp. 26123-8, 1995 (PubMed).

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