Growth Factor Receptor-Bound Protein 2 (GRB2) antibody

Antigen: Growth Factor Receptor-Bound Protein 2 (GRB2)

Synonyms: ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, MGC108668, zgc:73077, wu:fb18e12, wu:fb34e06, GRB2, MGC127576, DKFZp459P153, grb2, MGC64880, BB233594, 8430435N19Rik, MGC112733, GRAP, MGC166297, MGC110202, zgc:110202, MGC109892, si:dkeyp-9d4.1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Catalog no.: ABIN184670

Additional Information

Alternative name: GRB2

Gene ID: 2885, 14784 (mouse), 81504 (rat)

Immunogen: C-PRNYVTPVNRNV

Format: Affinity-purified

Description: All names and symbols: GRB2, growth factor receptor-bound protein 2, ASH, ASH protein, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, HT027, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, growth factor receptor-bound protein 3. Official Symbol: GRB2. RefSeq number(s): NP_002077.1, NP_987102.1. Gene ontology terms: SH3/SH2 adaptor protein activity, EGF receptor signaling pathway, RAS protein signal transduction, intracellular signaling cascade, cell-cell signaling

Specificity: This antibody is expected to recognize both reported isoforms (NP_002077.1and NP_987102.1). Human, Mouse, Rat, Dog, Zebrafish 217 aa

Synonyms: ASH, Grb3-3, MST084, NCKAP2, MSTP084, EGFRBP-GRB2, Ash, AA408164, MGC108668, zgc:73077, wu:fb18e12, wu:fb34e06, GRB2, MGC127576, DKFZp459P153, grb2, MGC64880, BB233594, 8430435N19Rik, MGC112733, GRAP, MGC166297, MGC110202, zgc:110202, MGC109892, si:dkeyp-9d4.1

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution >1:32,000. Western blot: Approx 30kDa band seen in Jurkat lysate. Recommended for use at 1-3µg/ml. Immunohistochemistry: In paraffin embedded Human Tonsil, stains mantle zone and to a lessser extent germinal centre. Recommended concentration, 0.1-0.3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Cancer

Restrictions: For Research Use only

Images

ABIN184670 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ABIN184670 (0.3µg/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH6, HRP-staining.

Publications

Publications: Lowenstein, Daly, Batzer et al.: "The SH2 and SH3 domain-containing protein GRB2 links receptor tyrosine kinases to ras signaling." in: Cell, Vol. 70, Issue 3, pp. 431-42, 1992 (PubMed).