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Src Kinase Associated Phosphoprotein 2 (SKAP2) (C-Term) antibody

Details for Product No. ABIN184706, Supplier: Log in to see
Antigen
  • scap2
  • 2610021A10Rik
  • AA960083
  • BB137539
  • RA70
  • SKAP-HOM
  • Saps
  • Scap2
  • mSKAP55R
  • PRAP
  • SAPS
  • SCAP2
  • SKAP55R
  • Scap55r
  • SKAP55
  • si:dz227h09.6
  • si:dz31b14.4
  • zgc:158223
  • zgc:65975
Epitope
C-Term
15
14
14
11
3
2
2
2
1
1
1
1
Reactivity
Human
64
19
19
3
3
1
1
Host
Goat
46
15
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
ELISA, Western Blotting (WB)
49
31
11
7
6
4
3
1
1
1
Supplier
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Immunogen Peptide with sequence C-GLVPKAYIMEMYDI, from the C Terminus of the protein sequence according to NP_003921.2.
Blocking Peptide Blocking peptide for this product available: ABIN369126
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name SCAP2 / PRAP (SKAP2 Antibody Abstract)
Background Synonyms: SKAP2, SCAP2, PRAP, src family associated phosphoprotein 2, MGC10411, MGC33304, RA70, SAPS, SCAP2, SKAP-HOM, SKAP55R, Fyn-associated phosphoprotein SKAP55 homologue, Pyk2/RAFTK-associated protein, src family associated phosphoprotein 2, src kinase-associated phosphoprotein of 55-related protein, src-associated adaptor protein
Gene ID 8935
NCBI Accession NP_003921
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 38 kDa band observed in lysates of cell line Jurkat (calculated MW of 41.2 kDa according to NP_003921.2). In transfected HEK293 transiently expressing SKAP2 a band of approx. 45 kDa is observed. This band is not observed in the non-transfected HEK293. The calculated Recommended concentration: 0.5-2 µg/mL. An additional band of unknown identity was also consistently observed in Jurkat at 28 kDa. This band was successfully blocked by incubation with the immunising peptide.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
 image for anti-Src Kinase Associated Phosphoprotein 2 (SKAP2) (C-Term) antibody (ABIN184706) ABIN184706 (0.5µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Primar...
Background publications Park, Avraham, Avraham: "RAFTK/Pyk2 activation is mediated by trans-acting autophosphorylation in a Src-independent manner." in: The Journal of biological chemistry, Vol. 279, Issue 32, pp. 33315-22, 2004 (PubMed).

Takahashi, Yamashita, Nagano et al.: "Identification and characterization of a novel Pyk2/related adhesion focal tyrosine kinase-associated protein that inhibits alpha-synuclein phosphorylation." in: The Journal of biological chemistry, Vol. 278, Issue 43, pp. 42225-33, 2003 (PubMed).