IRF5 antibody (C-Term)

Catalog No. ABIN184812

This anti-IRF5 antibody is a Goat Polyclonal antibody detecting IRF5 in WB and ELISA. Suitable for Human.

Quick Overview for IRF5 antibody (C-Term) (ABIN184812)

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This IRF5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-IRF5 Antibody

Binding Specificity: C-Term

Purpose: IRF5

Sequence: QGPWPMHPAG MQ

Specificity: This antibody is expected to recognize reported isoforms (NP_116032.1, NP_001092097.2 , NP_001092099.1

Cross-Reactivity: Cow, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-QGPWPMHPAGMQ, from the C Terminus of the protein sequence according to NP_002191.1, NP_116032.1, NP_001092097.1, NP_001092099.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 55+60 kDa bands observed in Human Spleen lysates and approx 65 kDa in lysates of cell line A549 (calculated MW of 57.8 kDa according to NP_001092099.1 and 56.0 kDa according to NP_116032.1, NP_001092097.2). Recommended concentration: 0.3

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for IRF5

Target: IRF5 (Interferon Regulatory Factor 5 (IRF5))

Alternative Name: IRF5

Background: IRF5, interferon regulatory factor 5, SLEB10

Gene ID: 3663

NCBI Accession: NP_116032, NP_001092097, NP_001092099

Pathways: TLR Signaling, Autophagy