Interferon Regulatory Factor 8 (IRF8) (C-Term) antibody

Details for Product No. ABIN184813
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Antigen
Synonyms zgc:92252, IRF8, MGC140338, DKFZp469B0320, H-ICSBP, ICSBP, ICSBP1, IRF-8, icsbp1, AI893568, Icsbp1, Myls
Epitope
C-Term
(23), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(48), (27), (19), (4), (3), (2), (2), (2)
Host
Goat
(46), (7), (1)
Clonality
Polyclonal
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB)
(50), (22), (9), (8), (6), (5), (1)
Pubmed 1 reference available
Quantity 100 µg
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Catalog No. ABIN184813
374.27 $
Plus shipping costs $45.00

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Immunogen Peptide with sequence C-QRSFFRENQQITV, from the C Terminus of the protein sequence according to NP_002154.1.
Cross-Reactivity Mouse (Murine), Rat (Rattus), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name ICSBP1 / IRF8
Background Synonyms: IRF8, ICSBP1, interferon consensus sequence binding protein 1, IRF-8, H-ICSBP, interferon regulatory factor 8
Gene ID 3394, 15900
NCBI Accession NP_002154
Application Notes ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 45-50 kDa band seen in K562 lysate (Predicted MW of 47 kDa according to NP_002154). Recommended for use at 0.5-2 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Interferon Regulatory Factor 8 (IRF8) (C-Term) antibody ABIN184813 staining (1µg/ml) of K562 lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
General Weisz, Marx, Sharf et al.: "Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon-inducible genes." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 25589-96, 1993 (PubMed).

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