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Details for Product No. ABIN184880

Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2) (N-Term) antibody

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Synonyms AAD10, ALR, CAGL114, KABUK1, KMS, MLL2, MLL4, TNRC21, BC032281, BC058659, C430014K11Rik, KMT2B, Mll2, Mll4, im:7157663, mll2
»Alternatives N-Term
»Alternatives Human
Clonality Polyclonal
»Alternatives Un-conjugated
Methode Type Peptide ELISA
»Alternatives ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 1 reference available
Catalog no. ABIN184880
Quantity 100 µg
374.27 $   Plus shipping costs $45.00
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Immunogen Peptide with sequence DSQNLAGEDKDSQ-C, from the N Terminus of the protein sequence according to NP_003473.3.
Specificity The immunizing peptide was designed from NP_003473.1. Since then the sequence has changed in the databases and currently differs at two locations in NP_003473.3.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MLL2
Background Synonyms: MLL2, myeloid/lymphoid or mixed-lineage leukemia 2, ALR, ALL1-related gene, AAD10, CAGL114, KMT2B, MLL4, TNRC21, ALL1-related, trinucleotide repeat containing 21
Gene ID 8085
NCBI Accession NP_003473.3
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave bands at approx 75 kDa and 25 kDa in lysates of cell line K562 after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 593 kDa according to NP_003473.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Placenta shows nuclear stainng in the trophoblasts . Similar nuclear patches are observed in Human Liver. Recommended concentration: 4-8 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Prasad, Zhadanov, Sedkov et al.: "Structure and expression pattern of human ALR, a novel gene with strong homology to ALL-1 involved in acute leukemia and to Drosophila trithorax." in: Oncogene, Vol. 15, Issue 5, pp. 549-60, 1997 (PubMed).

Alternatives for antigen "Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2)", type "Antibodies"
Hosts (15), (7), (1)
Reactivities (22), (13), (12), (12), (12), (12)
Applications (13), (10), (7), (6), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (3), (1)