Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2) (N-Term) antibody

Details for Product No. ABIN184880
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Antigen
Synonyms im:7157663, mll2, GSTO1, ALR, HRX2, MLL1B, MLL2, MLL4, TRX2, WBP7, BC032281, BC058659, C430014K11Rik, KMT2B, Mll2, Mll4, AAD10, CAGL114, KABUK1, KMS, TNRC21
Epitope
N-Term
(8), (6), (4), (1), (1), (1), (1), (1)
Reactivity
Human
(33), (13), (12), (12), (12), (12)
Host
Goat
(25), (7), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
(19), (10), (8), (7), (1)
Pubmed 1 reference available
Quantity 100 µg
Options
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Catalog No. ABIN184880
374.27 $
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Immunogen Peptide with sequence DSQNLAGEDKDSQ-C, from the N Terminus of the protein sequence according to NP_003473.3.
Specificity The immunizing peptide was designed from NP_003473.1. Since then the sequence has changed in the databases and currently differs at two locations in NP_003473.3.
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name MLL2
Background Synonyms: MLL2, myeloid/lymphoid or mixed-lineage leukemia 2, ALR, ALL1-related gene, AAD10, CAGL114, KMT2B, MLL4, TNRC21, ALL1-related, trinucleotide repeat containing 21
Gene ID 8085
NCBI Accession NP_003473
Application Notes ELISA: antibody detection limit dilution 1:16000.
Western blot: Preliminary experiments gave bands at approx 75 kDa and 25 kDa in lysates of cell line K562 after 0.1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 593 kDa according to NP_003473.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
IHC: In paraffin embedded Human Placenta shows nuclear stainng in the trophoblasts . Similar nuclear patches are observed in Human Liver. Recommended concentration: 4-8 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
anti-Myeloid/lymphoid Or Mixed-Lineage Leukemia 2 (MLL2) (N-Term) antibody (4µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.
General Prasad, Zhadanov, Sedkov et al.: "Structure and expression pattern of human ALR, a novel gene with strong homology to ALL-1 involved in acute leukemia and to Drosophila trithorax." in: Oncogene, Vol. 15, Issue 5, pp. 549-60, 1997 (PubMed).

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