UBE2K antibody (C-Term)

Catalog No. ABIN184891

This Goat Polyclonal antibody specifically detects UBE2K in ELISA, WB and IHC. It exhibits reactivity toward Human.

Quick Overview for UBE2K antibody (C-Term) (ABIN184891)

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2K antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-UBE2K Antibody

Binding Specificity: C-Term

Purpose: HIP2

Sequence: SWDVETATEL LLSN

Specificity: This antibody is expected to recognise isoform 1 (NP_005330.1), isoform 2 (NP_001104582.1) and isoform 3 (NP_001104583.1).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-SWDVETATELLLSN, from the C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain shows pixulate cytoplasm stainng in neurons of the Cortex. Recommended concentration, 1-2 μg/mL.

Western Blot: Approx 24 kDa band observed in Jurkat cell lysates (calculated MW of 22.4 kDa according to NP_005330). Recommended for use at 1-3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:2000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for UBE2K

Target: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Alternative Name: UBE2K

Background: UBE2K, HIP2, huntingtin interacting protein 2, LIG, HYPG, ubiquitin-protein ligase, ubiquitin carrier protein, ubiquitin-conjugating enzyme E2-25 KDA, ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast), DKFZp564C1216, DKFZp686J24237, E2-25K,

Gene ID: 3093

NCBI Accession: NP_005330, NP_001104582, NP_001104583