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Antibodies
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anti-Checkpoint with Forkhead and Ring Finger Domains (CHFR) antibody
Checkpoint with Forkhead and Ring Finger Domains (CHFR) antibody
| Antigen |
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| Synonyms |
CHFR, RNF116, RNF196, FLJ10796, FLJ33629, chfr, fc43f10, wu:fc43f10, MGC95316, rnf116, rnf196, MGC75959, DKFZp469H0121, C230082M18, 5730484M20Rik |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Application
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| Catalog no. |
ABIN184918 |
| Quantity |
100 ug |
| Price |
501.80 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 Business Days |
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Alternative name
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CHFR
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Gene ID
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55743, 231600 (mouse), 288734 (rat)
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Immunogen
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KFNHICEQTRFKN
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Format
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Affinity-purified
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Description
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All names and symbols: CHFR, checkpoint with forkhead and ring finger domains, FLJ10796. Official Symbol: CHFR. RefSeq number(s): NP_060693
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Specificity
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Human, Mouse, Rat
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Application Notes
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Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Preliminary experiments gave an approx 80kDa band in human brain lysates at 0.5ug/ml, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 72kDda according to NP_060693. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded human brain (cerebellum) shows stained Purkinje cells. Recommended concentration, 2.5µg/ml.
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Purification
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Buffer
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0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Storage
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Aliquot and store at -20°C. Minimize freezing and thawing.
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Research Area
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Cancer, Cell Cycle
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Restrictions
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For Research Use only
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Publications
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Scolnick, Halazonetis: "Chfr defines a mitotic stress checkpoint that delays entry into metaphase." in: Nature, Vol. 406, Issue 6794, pp. 430-5, 2000 (PubMed).
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Alternatives for antigen "Checkpoint with Forkhead and Ring Finger Domains (CHFR)", type "Antibodies"
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Hosts
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Rabbit (22), Mouse (6), Goat (2)
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Reactivities
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Human (28), Mouse (Murine) (19), Rat (Rattus) (4), Chicken (3), Cow (Bovine) (2), Dog (Canine) (2)
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Applications
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Flow Cytometry (FACS) (18), Immunofluorescence (IF) (18), Western Blotting (WB) (10), ELISA (7), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (7), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), Immunoprecipitation (IP) (2), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1)
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Alternatives 
