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CHFR antibody (Checkpoint with Forkhead and Ring Finger Domains) (AA 1-652) Primary Antibody

CHFR Reactivity: Human IHC (p), RNAi, ELISA, WB Host: Mouse Monoclonal 1H3-A12 unconjugated
Pubmed (7)
Catalog No. ABIN565913
$600.00
Plus shipping costs $45.00
100 μg
local_shipping Shipping to: United States
Delivery in 11 to 12 Business Days
  • Target
    CHFR
    Binding Specificity
    • 15
    • 7
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 1-652
    Reactivity
    • 50
    • 14
    • 13
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 40
    • 7
    • 3
    Mouse
    Clonality
    • 48
    • 2
    Monoclonal
    Conjugate
    • 15
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CHFR antibody is un-conjugated
    Application
    • 34
    • 28
    • 14
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), RNA Interference (RNAi), ELISA, Western Blotting (WB)
    Purpose
    Mouse monoclonal antibody raised against a full length recombinant CHFR.
    Cross-Reactivity
    Human
    Immunogen

    immunogen: CHFR (AAH12072.1, 1 a.a. ~ 652 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

    Immunogen Sequence: MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRVEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN

    Clone
    1H3-A12
    Isotype
    IgG1 kappa
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    Product Quality tested by: Antibody Reactive Against Recombinant Protein.

    Restrictions
    For Research Use only
  • Buffer
    In 1x PBS, pH 7.4
    Handling Advice
    Aliquot to avoid repeated freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Fujita, Yamamoto, Imamura, Nakamura, Maehara, Tsuneyoshi, Oda: "Expression level of the mitotic checkpoint protein and G2-M cell cycle regulators and prognosis in gastrointestinal stromal tumors in the stomach." in: Virchows Archiv : an international journal of pathology, Vol. 460, Issue 2, pp. 163-9, 2012 (PubMed).

    Kashima, Idogawa, Mita, Shitashige, Yamada, Ogi, Suzuki, Toyota, Ariga, Sasaki, Tokino: "CHFR protein regulates mitotic checkpoint by targeting PARP-1 protein for ubiquitination and degradation." in: The Journal of biological chemistry, Vol. 287, Issue 16, pp. 12975-84, 2012 (PubMed).

    Hu, Huang, Sun, Wu, Xu, Yin, Chen, Jiang, Shen: "Pathobiologic implications of methylation and expression status of Runx3 and CHFR genes in gastric cancer." in: Medical oncology (Northwood, London, England), Vol. 28, Issue 2, pp. 447-54, 2011 (PubMed).

    Privette, Weier, Nguyen, Yu, Petty: "Loss of CHFR in human mammary epithelial cells causes genomic instability by disrupting the mitotic spindle assembly checkpoint." in: Neoplasia (New York, N.Y.), Vol. 10, Issue 7, pp. 643-52, 2008 (PubMed).

    Gao, Xin, Zhang, Zhou: "Mechanism and pathobiologic implications of CHFR promoter methylation in gastric carcinoma." in: World journal of gastroenterology : WJG, Vol. 14, Issue 32, pp. 5000-7, 2008 (PubMed).

    Milne, Sitarz, Carvalho, Polak, Ligtenberg, Pauwels, Offerhaus, Weterman: "Molecular analysis of primary gastric cancer, corresponding xenografts, and 2 novel gastric carcinoma cell lines reveals novel alterations in gastric carcinogenesis." in: Human pathology, Vol. 38, Issue 6, pp. 903-13, 2007 (PubMed).

    Privette, González, Ding, Kleer, Petty: "Altered expression of the early mitotic checkpoint protein, CHFR, in breast cancers: implications for tumor suppression." in: Cancer research, Vol. 67, Issue 13, pp. 6064-74, 2007 (PubMed).

  • Target
    CHFR
    Alternative Name
    CHFR (CHFR Antibody Abstract)
    Synonyms
    RNF116, RNF196, CHFR, rnf116, rnf196, 5730484M20Rik, C230082M18, checkpoint with forkhead and ring finger domains, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog, CHFR, chfr, Chfr, chfr.L
    Background
    Full Gene Name: checkpoint with forkhead and ring finger domains
    Synonyms: FLJ10796,FLJ33629,RNF116,RNF196
    Gene ID
    55743
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