Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

CHFR antibody (Checkpoint with Forkhead and Ring Finger Domains) (N-Term) Primary Antibody

CHFR Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio) IHC, WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN2774757
$389.00
Plus shipping costs $45.00
100 μL
local_shipping Shipping to: United States
Delivery in 2 to 3 Business Days
  • Target
    CHFR
    Binding Specificity
    • 15
    • 7
    • 7
    • 4
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish (Danio rerio)
    Host
    • 39
    • 7
    • 4
    Rabbit
    Clonality
    • 47
    • 3
    Polyclonal
    Conjugate
    • 15
    • 6
    • 4
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    This CHFR antibody is un-conjugated
    Application
    • 35
    • 28
    • 13
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Western Blotting (WB)
    Sequence
    REWTIGRRRG CDLSFPSNKL VSGDHCRIVV DEKSGQVTLE DTSTSGTVIN
    Predicted Reactivity
    Cow: 93%, Dog: 93%, Guinea Pig: 100%, Horse: 93%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 86%
    Characteristics
    This is a rabbit polyclonal antibody against CHFR. It was validated on Western Blot using a cell lysate as a positive control.
    Purification
    Affinity Purified
    Immunogen
    The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR
  • Application Notes
    Optimal working dilutions should be determined experimentally by the investigator.
    Comment

    Antigen size: 623 AA

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
  • Target
    CHFR
    Alternative Name
    CHFR (CHFR Antibody Abstract)
    Synonyms
    RNF116, RNF196, CHFR, rnf116, rnf196, 5730484M20Rik, C230082M18, checkpoint with forkhead and ring finger domains, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase, checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase L homeolog, CHFR, chfr, Chfr, chfr.L
    Background
    CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.
    Alias Symbols: FLJ10796, FLJ33629, RNF116, RNF196
    Protein Interaction Partner: USP7, VIM, UBE2D2, AURKA, HLTF, RANBP2, PLK1, PCNA, MCM2, HDAC2, HDAC1, CDK5, CDC20, PARP1, SIN3A, CHFR, UBC13, SUMO1, UBE2I, LRP1, UBE2V1, UBE2N, UBE2D1, TRIM9, RNF125, CBLC, RNF7, RELA, RNF8, HIST2H2BE, HIST2H2AC, UBC, SMARCD1, SMARCB1, SMARCA4, KIF22,
    Protein Size: 623
    Molecular Weight
    69 kDa
    Gene ID
    55743
    NCBI Accession
    NM_018223, NP_060693
    UniProt
    Q96EP1
You are here:
help Support