Endonuclease VIII-Like 1 (NEIL1) antibody

Antigen: Endonuclease VIII-Like 1 (NEIL1)

Synonyms: FPG1, NEI1, hFPG1, FLJ22402

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN184936

Additional Information

Alternative name: NEIL1 / NEH1

Gene ID: 79661

Immunogen: C-DIPSLEPEGTSAS

Format: Affinity-purified

Description: All names and symbols: NEIL1, NEH1, nei endonuclease VIII-like 1 (E. coli), NEI1, FLJ22402, endonuclease VIII. Official Symbol: NEIL1. RefSeq number(s): NP_078884.1. Gene ontology terms: DNA repair, endonuclease activity

Specificity: Human

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:16,000. Western Blot: Preliminary experiments gave an approx 60kDa band in Human Liver and Brain lysates at 1ug/ml. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 43.7kDda according to NP_078884.1. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Placenta shows strong staining of cytoplasm in syncytiotrophoblasts. Recommended concentration, 5-10µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Restrictions: For Research Use only

Images

ABIN184936 (5µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Publications

Publications: Hazra, Izumi, Boldogh et al.: "Identification and characterization of a human DNA glycosylase for repair of modified bases in oxidatively damaged DNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 6, pp. 3523-8, 2002 (PubMed).