NEIL1 antibody (Internal Region)

Catalog No. ABIN6258289

Product Details anti-NEIL1 Antibody

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Binding Specificity: Internal Region

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEIL1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: NEIL1 Antibody detects endogenous levels of total NEIL1.

Predicted Reactivity: Horse,Sheep

Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink^TM Coupling Resin (Thermo Fisher Scientific).

Immunogen: A synthesized peptide derived from human NEIL1, corresponding to a region within the internal amino acids.

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:1000, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Rabbit IgG in phosphate buffered saline , pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20 °C. Stable for 12 months from date of receipt.

Expiry Date: 12 months

Target Details for NEIL1

Target: NEIL1 (Endonuclease VIII-Like 1 (NEIL1))

Alternative Name: NEIL1 (NEIL1 Products)

Synonyms: FPG1 antibody, NEI1 antibody, hFPG1 antibody, 2810450N13Rik antibody, Nei1 antibody, nei-like DNA glycosylase 1 antibody, nei like DNA glycosylase 1 antibody, nei endonuclease VIII-like 1 (E. coli) antibody, Neil1 antibody, NEIL1 antibody

Background:

Description: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, formamidopyrimidine (Fapy) and 5-hydroxyuracil. Has marginal activity towards 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Has DNA glycosylase/lyase activity towards mismatched uracil and thymine, in particular in U:C and T:C mismatches. Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting that it acts as a specific reader of 5hmC.

Gene: NEIL1

Molecular Weight: 44 kDa

Gene ID: 79661

UniProt: Q96FI4

Pathways: DNA Damage Repair