Alstrom Syndrome 1 (ALMS1) antibody

Antigen: Alstrom Syndrome 1 (ALMS1)

Synonyms: ALSS, KIAA0328, DKFZp686A118, DKFZp686D1828, bbb, ALMS1

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: ELISA, Immunohistochemistry (IHC)

Catalog no.: ABIN184952

Additional Information

Alternative name: ALMS1

Gene ID: 7840, 236266 (mouse), 297408 (rat)

Immunogen: C-RVTNQLLGRKVPWD

Format: Affinity-purified

Description: All names and symbols: ALMS1, Alstrom syndrome 1, ALSS, KIAA0328. Official Symbol: ALMS1. RefSeq number(s): NP_055935.3

Specificity: The C terminus of ALMS1 shares an 8 amino acid stretch with two other human proteins: hypothetical protein (XP_169104) and ERAL1 (NP_005693). However, this stretch is located internally in these two proteins and so will most likely not cross-react with this antibody. The N terminus was not chosen as the peptide is expected to cyclise during synthesis. Human, Mouse, Rat, Dog

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Preliminary experiments gave bands at approx 200kDa and 75kDa in lysates of Human T cell line Jurkat after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kDa according to NP_055935.3. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? Immunohistochemistry: In paraffin embedded Human Testis shows staining of cytoplasm in spermatocytes. Recommended concentration, 1-3µg/ml.

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Buffer: 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

Storage: Aliquot and store at -20°C. Minimize freezing and thawing.

Research Area: Immunology

Restrictions: For Research Use only

Images

ABIN184952 (2µg/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

Publications

Publications: Collin, Marshall, Ikeda et al.: "Mutations in ALMS1 cause obesity, type 2 diabetes and neurosensory degeneration in Alström syndrome." in: Nature genetics, Vol. 31, Issue 1, pp. 74-8, 2002 (PubMed).