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Ubiquitin Protein Ligase E3 Component N-Recognin 5 (UBR5) (C-Term) antibody

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Antigen
Synonyms DD5, EDD, EDD1, HYD, 4432411E13Rik, AW549941, C77315, D030042K14, Edd, Edd1, mKIAA0896, Dd5, Rat100
Epitope
C-Term
(32), (8), (4), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Rat (Rattus), Mouse (Murine), Rabbit
(78), (34), (19), (7), (6), (3), (2), (2), (1), (1)
Host
Goat
(55), (20), (3), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(4), (4), (4), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Methode Type Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(43), (40), (38), (16), (11), (6), (3), (2)
Pubmed 1 reference available
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Quantity 100 μg
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Immunogen Peptide with sequence C-LAIKTKNFGFV, from the C Terminus of the protein sequence according to NP_056986.2.
Blocking Peptide Blocking peptide for this product available: ABIN369399
Cross-Reactivity Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name EDD1 / HYD (UBR5 Antibody Abstract)
Background Synonyms: UBR5, EDD1, E3 ubiquitin protein ligase, HECT domain containing, 1, DD5, EDD, HYD, KIAA0896, progestin induced protein, ubiquitin-protein ligase, E3 identified by differential display, hyperplastic discs protein homolog, ubiquitin protein ligase E3 component n-recognin 5, FLJ11310, MGC57263
Molecular Weight 322kDa
Gene ID 51366, 70790, 117060
NCBI Accession NP_056986
Pathways
Application Notes ELISA: antibody detection limit dilution 1:4000.
Western blot: An anonymous customer found positive results in WB on Human breast cancer cells (MDA-MB-468). An anonymous customer found positive results in WB on Human HeLa cells. An anonymous customer found positive results in WB on Human glioblastoma (T98G cells). An anonymous customer found positive results in WB on Rat glioma (C6 cells). An anonymous customer found positive results in WB on Mouse fibroblasts (NIH 3T3). An anonymous customer found positive results in WB on Rabbit heart tissue.
IHC: In paraffin embedded Human Testis shows nuclear staining in . Recommended concentration: 1-2 µg/mL. Immunoprecipitation: An anonymous customer found positive results in IP on Human breast cancer cells. IP, An anonymous customer found positive results in IP on Mouse lung tissue. IP, An anonymous customer found positive results in IP on Rat lung tissue. IP, An anonymous customer found positive results in IP on Rabbit heart tissue.
Restrictions For Research Use only
Format Liquid
Concentration 100µg/200µl
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Henderson, Russell, Hird et al.: "EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response." in: The Journal of biological chemistry, Vol. 277, Issue 29, pp. 26468-78, 2002 (PubMed).

Catalog No. ABIN184976
$318.20
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