IFIH1 antibody (N-Term)

Catalog No. ABIN185002

This Goat Polyclonal antibody specifically detects IFIH1 in WB, ELISA, IHC and FACS. It exhibits reactivity toward Human and Monkey and has been mentioned in 3+ publications.

Quick Overview for IFIH1 antibody (N-Term) (ABIN185002)

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Reactivity: Human, Monkey

Host: Goat

Clonality: Polyclonal

Conjugate: This IFIH1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Grade: Verified

Product Details anti-IFIH1 Antibody

Binding Specificity: N-Term

Purpose: MDA5 / IFIH1

Sequence: SNGYSTDENF RYL

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence SNGYSTDENFRYL-C, from the N Terminus of the protein sequence according to NP_071451.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Pancreas, shows strong cytoplasmic staining of some cells in the islets of Langerhans. Recommended concentration: 10 μg/mL.

Western Blot: This antibody has been successfully used in Western blot on African Green Monkey: Sato S et al, Arthritis Rheum. 2009 Jul,60(7):2193-200. PMID: 19565506.

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-IFIH1 antibody (ABIN185002)

Sato, Hoshino, Satoh, Fujita, Kawakami, Fujita, Kuwana et al.: "RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial ..." in: Arthritis and rheumatism, Vol. 60, Issue 7, pp. 2193-200, (2009) (PubMed).

Liao, Bao, Liu, Lai, Li, Garofalo, Casola: "Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling." in: The Journal of general virology, Vol. 89, Issue Pt 8, pp. 1978-86, (2008) (PubMed).

Bao, Sinha, Liu, Hong, Luxon, Garofalo, Casola: "Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis." in: Virology, Vol. 374, Issue 1, pp. 114-27, (2008) (PubMed).

Target Details for IFIH1

Target: IFIH1 (Interferon Induced with Helicase C Domain 1 (IFIH1))

Alternative Name: IFIH1

Background: IFIH1, MDA5, MDA-5, Hlcd, MGC133047, IDDM19, melanoma differentiation associated protein-5, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide, interferon induced with helicase C domain 1, helicard

Gene ID: 64135

NCBI Accession: NP_071451

Pathways: Activation of Innate immune Response