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FOXI1 antibody (Forkhead Box I1) (C-Term)

Details for Product anti-FOXI1 Antibody No. ABIN185038, Supplier: Log in to see
Antigen
  • 5830401E05Rik
  • cb724
  • ema
  • Fkh10
  • FKH10
  • FKHL10
  • fkhl10
  • foo
  • FOXI1
  • foxi1a
  • FoxI1e
  • foxi1e
  • FREAC-6
  • freac6
  • FREAC6
  • HFH-3
  • HFH3
  • Hfh3
  • hfh3
  • xema
  • Xema
  • xfoxi1
  • Xfoxi1a
Epitope
C-Term
17
16
12
10
9
4
3
3
2
1
1
1
1
1
Reactivity
Mouse (Murine)
91
37
17
4
2
1
1
1
1
1
Host
Goat
61
26
15
Clonality
Polyclonal
Conjugate
This FOXI1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
64
46
31
22
16
13
11
2
2
Supplier
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Immunogen Peptide with sequence C-TSGVLYPREGTEV, from the C Terminus of the protein sequence according to NP_036320.2, NP_658982.1.
Blocking Peptide Blocking peptide for this product available: ABIN369991
Specificity This antibody is expected to recognise both reported human isoforms (NP_036320.2, NP_658982.1).
Cross-Reactivity Human
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name FOXI1 / FKHL10 (FOXI1 Antibody Abstract)
Background Synonyms: FOXI1, forkhead box I1, HFH3, FKHL10, FREAC6, HNF-3/fork-head homolog-3, forkhead-related activator 6, forkhead (Drosophila)-like 10, Forkhead, drosophila, homolog-like 10, hepatocyte nuclear factor 3 forkhead homolog 3, FKH10, MGC34197, forkhead-like 10
Gene ID 2299
NCBI Accession NP_658982, NP_036320
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave bands at approx 50 kDa and 25 kDa in Human Kidney lysate after 1 µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the predicted sizes of 41.0 kDa according to NP_036320 (isoform a) and 30.8 kDa according to NP_65892 (isofrom b). The bands were successfully blocked by incubation with the immunizing peptide.
IHC: This product has been successfully used in Mouse Epididymus.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Background publications Pierrou, Hellqvist, Samuelsson, Enerbäck, Carlsson: "Cloning and characterization of seven human forkhead proteins: binding site specificity and DNA bending." in: The EMBO journal, Vol. 13, Issue 20, pp. 5002-12, 1994 (PubMed).