UBA2 antibody (N-Term)

Catalog No. ABIN185083

This Goat Polyclonal antibody specifically detects UBA2 in WB, ELISA and IF. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for UBA2 antibody (N-Term) (ABIN185083)

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This UBA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Grade: Verified

Product Details anti-UBA2 Antibody

Binding Specificity: N-Term

Purpose: SAE2 / UBA2

Sequence: ALSRGLPREL AEA

Cross-Reactivity: Cow, Human, Mouse

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: ALSRGLPRELAEA-C

Isotype: IgG

Application Details

Application Notes:

Western Blot: This product has been successfully used in Western blot on Mouse: Kanemaru A, Saitoh H., Biosci Biotechnol Biochem. 2013,77(7):1575-8. PMID: 23832333.

Peptide ELISA: antibody detection limit dilution 1:16000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS and HeLa cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-UBA2 antibody (ABIN185083)

Kanemaru, Saitoh: "High-yield expression of mouse Aos1-Uba2-fusion SUMO-activating enzyme, mAU, in a baculovirus-insect cell system." in: Bioscience, biotechnology, and biochemistry, Vol. 77, Issue 7, pp. 1575-8, (2013) (PubMed).

Target Details for UBA2

Target: UBA2 (SUMO1 Activating Enzyme Subunit 2 (UBA2))

Alternative Name: SAE2

Background: UBA2, SAE2, SUMO-1 activating enzyme subunit 2

Gene ID: 10054, 50995

NCBI Accession: NP_005490