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EIF4ENIF1 antibody (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1) (C-Term)

Details for Product anti-EIF4ENIF1 Antibody No. ABIN185154, Supplier: Log in to see
Antigen
  • RGD1560908
  • eif4enif1
  • MGC80355
  • EIF4ENIF1
  • wu:fd15a05
  • zgc:101646
  • MGC147579
  • 4E-T
  • Clast4
  • 2610509L04Rik
  • A930019J01Rik
  • AA410001
  • AU021239
  • D11Ertd166e
Epitope
C-Term
23
18
15
13
6
4
3
2
2
2
Reactivity
Human
85
18
18
4
4
4
1
1
1
1
Host
Goat
52
18
15
Clonality
Polyclonal
Conjugate
This EIF4ENIF1 antibody is un-conjugated
4
4
4
3
3
3
1
1
1
1
1
1
1
1
1
Methode Type
Peptide ELISA
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
66
39
15
11
10
9
3
3
3
1
Supplier
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Immunogen Peptide with sequence C-AKVISVDELEYRQ, from the C Terminus of the protein sequence according to NP_062817.2, NP_001157974.1.
Blocking Peptide Blocking peptide for this product available: ABIN368798
Specificity This antibody is expected to recognise isoform 1 (NP_062817.2) and isoform b (NP_001157974.1). Reported variants represent identical protein (NP_062817.2, NP_001157973.1).
Cross-Reactivity Mouse (Murine), Rat (Rattus), Dog (Canine), Pig (Porcine), Cow (Bovine)
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Alternative Name 4E-T / EIF4ENIF1 (EIF4ENIF1 Antibody Abstract)
Background Synonyms: EIF4ENIF1, 4E-T, Clast4, FLJ21601, 2610509L04Rik, eukaryotic translation initiation factor 4E nuclear import factor 1, eIF4E-transporter, FLJ26551, OTTHUMP00000063276
Gene ID 56478, 74203
NCBI Accession NP_062817, NP_001157974
Pathways
Application Notes ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 140-150 kDa band observed in 293 lysates (predicted size of approx. 108 kDa according to NP_062817.1, however our observation agrees with that of Dostie et al (see below) ). Recommended for use at 0.25-0.5 µg/mL
IHC: In paraffin embedded Human Pancreas shows strong staining of cytoplasm in acinar cells-. Recommended concentration: 5-10 µg/mL.
Restrictions For Research Use only
Format Liquid
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-EIF4ENIF1 antibody (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1) (C-Term) (ABIN185154) ABIN185154 staining (0.25µg/ml) of 293 lysate (RIPA buffer, 35µg total protein per la...
Immunohistochemistry (IHC) image for anti-EIF4ENIF1 antibody (Eukaryotic Translation Initiation Factor 4E Nuclear Import Factor 1) (C-Term) (ABIN185154) ABIN185154 (5µg/ml) staining of paraffin embedded Human Pancreas. Steamed antigen ret...
Background publications Dostie, Ferraiuolo, Pause et al.: "A novel shuttling protein, 4E-T, mediates the nuclear import of the mRNA 5' cap-binding protein, eIF4E." in: The EMBO journal, Vol. 19, Issue 12, pp. 3142-56, 2000 (PubMed).