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TCP1 alpha/CCTA antibody (C-Term)

Verified TCP1 Reactivity: Human WB, ELISA, IF, IHC, FACS Host: Goat Polyclonal unconjugated
Catalog No. ABIN185185
  • Target See all TCP1 alpha/CCTA (TCP1) Antibodies
    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
    Binding Specificity
    • 57
    • 18
    • 17
    • 15
    • 15
    • 15
    • 9
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 147
    • 121
    • 58
    • 23
    • 21
    • 20
    • 18
    • 18
    • 17
    • 16
    • 13
    • 11
    • 10
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    Human
    Host
    • 128
    • 47
    • 19
    • 1
    Goat
    Clonality
    • 120
    • 75
    Polyclonal
    Conjugate
    • 68
    • 14
    • 12
    • 11
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This TCP1 alpha/CCTA antibody is un-conjugated
    Application
    • 146
    • 59
    • 59
    • 45
    • 42
    • 42
    • 40
    • 39
    • 25
    • 20
    • 11
    • 10
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)
    Purpose
    TCP1
    Sequence
    SYEDAVHSGA LND
    Specificity
    This antibody is expected to recognise both reported isoforms.
    Cross-Reactivity
    Human
    Purification
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    Grade
    Verified
    Immunogen
    Peptide with sequence C-SYEDAVHSGALND, from the C Terminus of the protein sequence according to NP_110379.2, NP_001008897.1.
    Isotype
    IgG
    Top Product
    Discover our top product TCP1 Primary Antibody
  • Application Notes

    Immunohistochemistry: In paraffin embedded Human Testis shows only cytoplasmic staining in some germ cells, and both cytoplasmic and nuclear staining in other germ cells with unstained nucleoli. Recommended concentration: 1-2 μg/mL.

    Western Blot: Approx 60 kDa band observed in lysates of cell line HEK293 and in Human Ovary lysates (calculated MW of 60.3 kDa according to NP_110379.2). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature.

    Peptide ELISA: antibody detection limit dilution 1:32000.

    Comment

    Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10μg/ml.

    Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    0.5 mg/mL
    Buffer
    Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Minimize freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
  • Target
    TCP1 alpha/CCTA (TCP1) (T-Complex 1 (TCP1))
    Alternative Name
    TCP1 (TCP1 Products)
    Synonyms
    CCT-alpha antibody, CCT1 antibody, CCTa antibody, D6S230E antibody, TCP-1-alpha antibody, AI528772 antibody, CCT antibody, Cct1 antibody, Ccta antibody, TRic antibody, Tcp-1 antibody, Tp63 antibody, c-cpn antibody, p63 antibody, TRiC antibody, CCTalpha antibody, BEST:GH05123 antibody, CG5374 antibody, Dmel\\CG5374 antibody, T-cpl antibody, TCP-1alpha antibody, TCPA_DROME antibody, Tcp1 antibody, Tcp1-alpha antibody, gh05123 antibody, cct-alpha antibody, ccta antibody, tcp1 antibody, tcp1-a antibody, tcp1a antibody, tcp1alpha antibody, CHUNP6875 antibody, fa13h08 antibody, wu:fa13h08 antibody, wu:fc95g06 antibody, t-complex 1 antibody, T-complex protein 1 subunit alpha antibody, t-complex protein 1 antibody, Tcp1-like antibody, t-complex 1 S homeolog antibody, TCP1 antibody, cct-1 antibody, Tcp1 antibody, T-cp1 antibody, tcp1.S antibody, tcp1 antibody
    Background
    TCP1, t-complex 1, CCT-alpha, CCT1, CCTa, D6S230E, TCP-1-alpha, T-complex protein 1, T-complex protein 1, alpha subunit, tailless complex polypeptide 1
    Gene ID
    6950
    NCBI Accession
    NP_110379, NP_001008897
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